单侧输尿管梗阻大鼠肾间质纤维化中氧化应激的变化木

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1、维普资讯http://www.cqvip.com第17卷第4期China中Jo国ur现na代lo医fM学od杂er志nMedicineV01．17No．42007年2月Feb．20o7文章编号：1005—8982(2007)04—0414—04·论著·单侧输尿管梗阻大鼠肾间质纤维化中氧化应激的变化木张晓暄，李银辉，郑伟，李根，那宇(吉林大学第四医院肾内科，吉林长春l30011)摘要：目的采用单侧输尿管结扎(UUO)致肾间质纤维化大鼠模型，观察UUO后肾脏病理和肾组织羟脯氨酸(Hyp)含量及各氧化应激指标的改变，探讨氧化应激在肾间质纤维化中的作用。方法成年健康Wist,

2、~大鼠24只，随机分为两组(n=12)：模型组(UUO组)大鼠麻醉后打开腹腔分离左侧输尿管，并于靠近肾盂段行左输尿管结扎；假手术组(Sham组)仅分离输尿管，不行结扎。两组大鼠于手术后10d处死取左侧肾脏，HE和Masson染色观察大鼠’肾脏常规病理；以化学比色法测定梗阻侧肾组织匀浆中羟脯氨酸(Hyp)的含量、总抗氧化能力(T-AOC)、总超氧化物歧化酶(T—SOD)和丙二醛(MDA)的含量。组间比较采用单因素方差分析，P

3、腔内有蛋白或细胞管型，间质趋向纤维化。与Sham组相比，UUO组梗阻侧肾脏病理改变明显，且肾组织中Hyp和MDA含量增加(P

4、脯氨酸；氧化应激中图分类号：R693文献标识码：AChangesofoxidationstressreactionininterstitialfibrosiskidneysofUUOratsZHANGXiao-xuan，LIYin—hui，ZHENGWei，LIGen，NAYu(DepartmentofNephrology,theFourthHospitalofJilinUniversity,Changchun,Jilin130011，P．R．China)Abstract：【Objective】Toobservethechangesofpathologyandcont

5、entsofhydroxyproline(Hyp)，totalantioxi-dizingcapability(T-AOC)，totalsuperoxidedismutase一SOD)andmalondialdehyde(MDA)inkidneysofunilateralureteralobstruction(UUO)rats，andinvestigatethefunctionofoxidationstressreactioninrenalinterstitialfibrosis．【Methods】24adulthealthyWistarratswererandoml

6、ydividedinto2groups(／1=l2)：ratsinUUOgroupunderwentleftunilateralureteralligationasdescribedpreviously；ratsinshamgrouphadtheiruretersmanipulatedbutnotlig-ated．Animalsweresacrificedatday10aftersurgery．Thehistologicalchangesinobstructedkidneytissuewereob-servedbyHEandMassonstains．Theconten

7、tsofHyp，T-AOC，T-SODandMDAweremeasuredbychemicalcol-orimetrymethod．DatawerepresentedasMean_+SD，statisticalanalysiswasperformedusingone-wayANOVA，P<0．05Wasconsideredsignificant．【Results】10dafterUUO，thetumafactionandatrophyinrenalparenchymaofob-structedkidneysweremeasuredobviously．