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时间:2020-04-25
《人胃癌SGC7901细胞株中干细胞对顺铂耐药的作用机制探讨-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、第23卷第17期China中Jo国ur现na代lof医M学od杂er志nMedicineV01.23No.172013年6月Jun.2013文章编号:1005—8982(2013)17—0025—05·论著·人胃癌SGC79O1细胞株中干细胞对顺铂耐药的作用机制探讨陈浩,黄烨,张克难,许浪z(1.湖北省荆州市中心医院胃肠外科,湖北荆州434000;2.武汉科技大学医学院,湖北武汉430074)摘要:目的探讨人胃癌SGC7901细胞中干细胞对顺铂耐药的作用机制。方法胃癌顺铂耐药细胞株sGc7901/DDP为实验组,胃癌SGC7901细胞为对照组,分别以
2、Hoechst33342染色法、免疫组织化学、Tran—swell小室检测实验组及对照组的sP细胞(sidepopulation,SP)比例、Nanog的表达及细胞侵袭力。结果实验组中细胞株侧群sP细胞的比例为(5.51±1.68)%,显著高于对照组(2.45±0.63)%(P<0.05);实验组中Nanog的阳性表达率为(79.06±10.37)%,显著高于对照组(55.18+3.64)%(P3、对实验组和对照组细胞的Ic值分别为(3.9±1.1)mol/L和(25.7±2.3)mol/L,实验组显著高于对照组(P<0.05)。结论胃癌干细胞可能与胃癌对顺铂耐药的机制密切相关,其中胃癌干细胞标志物sP细胞比例增高、Nanog基因高表达及细胞侵袭力增强发挥着重要作用。关键词:胃癌;干细胞;顺铂;SP细胞;耐药性中图分类号:IL735.2文献标识码:AMechanismonCisplatinresistanceofstemcellinhumangastriccancerSGC7901cellCHENHao,HUANGYe,ZHANGKe.nan,4、XULang.DepartmentofGastroenterology,CentralHospitalofJingzhou,Jingzhou,Hubei434000,P.R.China;2.MedicalSchoolofWuhanUniversityofScienceandTechnology,Wuhan,Hubei430074,P.R.China)Abstract:【Objective】ToinvestigatethemechanismonCisplatinresistanceofstemcellinhumangas-tilecancerSGC795、01cel1.【Methods】GastriccancerCisplatinresistantcel1lineSGC7901/DDPwasestab—lishedtobeasexperimentalgroup,andSGC7901cellwasascontrolgroup.ThepercentageofSPcellsinSGC7091celllinewasdetectedbyHoechst33342staining.ImmunocytochemicalstainingwasusedtodetecttheexpressionofNanog.Invasi6、oneffeetwasdetectedbyTranswell—system.【Results】,nleratioofSPcellsinexperimentalgroupwas(5.51±1.68).Itwassignificanthigherthanthatofcontrolgroup(2.45±O.63)%(P<0.05).TheexpressionofNanoginexperimentalgroupandcontrolgroupwere(79.06±10.37)%and(55.18+3.64)%respectively.Therehadasign7、ificantdifferencebetweenthetwogroups(P<0.05).Thenumberofcellspassedthroughfilminexperimentalgroupandcontrolgroupwere(65-I-7)and(41±6)respectively.Comparedwiththecontrolgroup,thenumberofcellspassedthroughfilminexperimentalgroupwassignifi—canflyhigherfP8、ateofgastriccancerinexperimentalgroupwassignificantlyh
3、对实验组和对照组细胞的Ic值分别为(3.9±1.1)mol/L和(25.7±2.3)mol/L,实验组显著高于对照组(P<0.05)。结论胃癌干细胞可能与胃癌对顺铂耐药的机制密切相关,其中胃癌干细胞标志物sP细胞比例增高、Nanog基因高表达及细胞侵袭力增强发挥着重要作用。关键词:胃癌;干细胞;顺铂;SP细胞;耐药性中图分类号:IL735.2文献标识码:AMechanismonCisplatinresistanceofstemcellinhumangastriccancerSGC7901cellCHENHao,HUANGYe,ZHANGKe.nan,
4、XULang.DepartmentofGastroenterology,CentralHospitalofJingzhou,Jingzhou,Hubei434000,P.R.China;2.MedicalSchoolofWuhanUniversityofScienceandTechnology,Wuhan,Hubei430074,P.R.China)Abstract:【Objective】ToinvestigatethemechanismonCisplatinresistanceofstemcellinhumangas-tilecancerSGC79
5、01cel1.【Methods】GastriccancerCisplatinresistantcel1lineSGC7901/DDPwasestab—lishedtobeasexperimentalgroup,andSGC7901cellwasascontrolgroup.ThepercentageofSPcellsinSGC7091celllinewasdetectedbyHoechst33342staining.ImmunocytochemicalstainingwasusedtodetecttheexpressionofNanog.Invasi
6、oneffeetwasdetectedbyTranswell—system.【Results】,nleratioofSPcellsinexperimentalgroupwas(5.51±1.68).Itwassignificanthigherthanthatofcontrolgroup(2.45±O.63)%(P<0.05).TheexpressionofNanoginexperimentalgroupandcontrolgroupwere(79.06±10.37)%and(55.18+3.64)%respectively.Therehadasign
7、ificantdifferencebetweenthetwogroups(P<0.05).Thenumberofcellspassedthroughfilminexperimentalgroupandcontrolgroupwere(65-I-7)and(41±6)respectively.Comparedwiththecontrolgroup,thenumberofcellspassedthroughfilminexperimentalgroupwassignifi—canflyhigherfP8、ateofgastriccancerinexperimentalgroupwassignificantlyh
8、ateofgastriccancerinexperimentalgroupwassignificantlyh
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