卵巢癌患者血浆miRNA水平与顺铂化疗敏感性的相关性研究-论文.pdf

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1、·930·中国医师杂志2014年7月第l6卷第7期JournalofChinesePhysician，July2014，Vo1．16。No．7论著·卵巢癌患者血浆miRNA水平与顺铂化疗敏感性的相关性研究冯朝霞黄余良欧阳先辉【摘要】目的探讨卵巢癌患者血浆miRNA分子标志物水平与铂类化疗敏感性的相关性。方法应用实时定量PCR方法检测48例卵巢癌患者化疗前后血浆中miRNA的表达，13例体检正常者为内参对照，观察卵巢癌患者铂类化疗药物的疗效及预后的关系。结果初检的11种miRNA中，仅有5种miRNA(miR．124，miR．

2、145，miR-22，miR一106a，miR-142)能够在外周血中检测出。其中3种miRNA(miR-22，miR一106a，miR一142)在正常人和卵巢癌患者的外周血中表达水平比较差异有统计学意义(P<0．O1)。患者化疗后外周血中仅miR-22水平显著升高(约3．2倍)，且miR-22水平高者对化疗反应多数敏感，反之不敏感。患者外周血miR一22的表达水平与其预后呈正相关(P<0．05)。结论血浆中miR-22，miR-106a，miR-142水平升高可能参与了卵巢癌的发生发展，化疗患者敏感者仅miR一22呈高表达

3、，不敏感者miR-22呈低表达，提示其可作为预测化疗敏感性及预后的指标之一。[关键词]卵巢肿瘤／药物疗法；微RNAs／血液；顺铂／治疗应用；预后StudyoftherelationshipbetweenmiRNAlevelinbloodplasmaofovariancancerpatientsandchemotherapysensitivitytocis-platinFengZhaoxia，HuangYuliang，OuyangXianhui．DepartmentofObstetricsandGynaecology，TheP

4、eople'sHospitalofHengnanCounty，Hengyang421101，ChinaCorrespondingauthor：HuangYuliang，Email：Liu661017@163．com【Abstract】ObjectiveToinvestigatethepotentialityofspecificmiRNAlevelinbloodplasmaofovariancancerpatientsasamolecularmarkertopredictsensitivityandresponsetoplat

5、inum—basedchemotherapy．MethodsQuantitativepolymerasechainreae—tion(Q-PCR)wasusedtocheckthechangingofmiRNAlevelinbloodplasmabeforeandafterchemotherapy，andexploredtherela—tivitybetweenvariationandchemotherapyresponseandclinicoutcome．ResultsAmongpreliminaryscreened11m

6、iRNAs，only5miRNAswereabletobedetectedinperipheralblood．Thereexistedvariationsin3miRNAs(miR-22，miR-106a，miR一142)．FurtherdetectionofthechangingofmiRNAlevelinbloodplasmabeforeandaftertherapy，miR-22wasf0undsignificantlyup-regulatedinbloodplasmathatwasunderwentplatinum—

7、basedchemotherapy．Meanwhile，ovariancancerpatientswithhighmiR一22levelweremost—lysensitivetoplatinum—basedtherapy．Otherwise，miR-22Waspositiverelativetotheclinicprognosisofpatientswithovariancancer．ConclusionsmiR-22，miR-106a，andmiR一142mightparticipateinthegenerationan

8、ddevelopmentofovariancancer．miR-22inpe—ripheralbloodwasprobablyidentifiedasanovelmolecularmarkerofpredictionofthesensitivitytoplatinuminovarianca