大肠杆菌氧化还原辅因子代谢工程-论文.pdf

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1、PROGRESSlNCHEMISTRYDOI．10．7536／PC140322大肠杆菌氧化还原辅因子代谢工程：f：王白云'王晓明''王智文陈涛'赵学明’(1．天津大学化工学院天津300072；2．系统生物工程教育部重点实验室天津300072；3．天津化学化工协同创新中心天津300072)摘要NAD、NADP、NADH、NADPH等四类烟酰胺类辅因子也被称为氧化还原辅因子，是维持细胞氧化还原平衡，驱动胞内许多分解与合成反应的重要分子。近年来，辅因子在生物转化系统中的作用受到研究者的重视，氧化还原辅因子更成为人们关注的重点。文章综述了

2、大肠杆菌(Escherichiacoli，E．coli)氧化还原辅因子代谢工程研究的最新进展，重点总结最近发展的氧化还原辅因子代谢工程策略，讨论不同代谢工程策略对细胞氧化还原辅因子水平的影响及其在生物合成中的应用，并展望了辅因子代谢工程未来发展方向。关键词氧化还原辅因子大肠杆菌代谢工程生物转化中图分类号：Q591；Q599文献标识码：A文章编号：1005—281X(2014)09．1609—10RedoxCofactorMetabolicEngineeringwithEscherichiacoliWangBaiyun,2,3tWa

3、ngXiaoyue,2,3tWangZhiwen，’ChenTao。’，ZhaoXueming·，(1．SchoolofChemicalEngineeringandTechnology，TianjinUniversity，Tianjin300072，China；2．KeyLaboratoryofSystemsBioengineering(MinistryofEducation)，TianjinUniversity，Tianjin300072，China；3．SynBioResearchPlatform，CollaborativeI

4、nnovationCenterofChemicalScienceandEngineering(Tianjin)，SchoolofChemicalEngineeringandTechnology，TianjinUniversity，Tianjin300072，China)AbstractRedoxcofactorplaysanimportantroleinmaintainingcellularredoxbalanceanddrivingcatabolicoranabolicreactions．Asthedrivingforceofb

5、iochemicalreactionsandredoxcarriers，redoxcofactorhasreceivedmuchattentionforenhancingbiotransformationprocessinrecentyears．TheGram—negativebacteriumEscherichiacoli(E．coli)hasbeenstudiedextensivelyonafundamentalandappliedlevelandhasbecomeapredominanthostmicroorganismfo

6、rindustrialapplications．MetabolicengineeringofE．colifortheenhancedbiochemicalproductionsuchasbioethanol，organicacids，biopolymer，complexnaturalcompoundsandsoon，hasbeensignificantlypromotedbytheredoxcofactorengineering．Thisreviewintroducedvariousstrategiestoimproveprodu

7、ctivityandproducttitersbyengineeredE．colithroughmetabolicengineeringpathwaysandkeyenzymesinvolvedredoxcofactor．AdvancedmetabolicengineeringstrategiesinredoxcofactorincludemetabolicengineeringofpathwayinvolvedinNAD(P)Hbiosynthesis，mutualtransformationofredoxcofactor，ex

8、pressionofheterogeneousredoxcofactordependentenzymes，manipulationofpyridinebiosynthesisandNADtransportation．Thesestrategiesh