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时间:2020-04-23
《不同来源致耐受DC对大鼠牙移植后牙根吸收及愈合影响的研究-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、重庆医学2014年9月第43卷第26期3461论著·基础研究doi:10.3969/j.issn.1671—8348.2014.26.019不同来源致耐受DC对大鼠牙移植后牙根吸收及愈合影响的研究邹林洪,张琳林,王豫蓉,单春城,明志强。,陈允嘉,李闻颖(1.重庆医科大学附属永川医院口腔科,重庆永川402160;2.重庆医科大学附属口腔医院正畸科400015)摘要:目的探讨不同来源致耐受树突细胞(DC)对大鼠同种异体牙移植后牙根吸收及愈合的影响。方法用BN、Lewis大鼠分别作为供、受体建立动物实验模型,采用完全随机法分为4组,每组
2、2O只:A为同基因移植;B、C、D为同种异体移植;A、B组于术前7d给大鼠输注PBS缓冲液0.5mL,C、D组于术前7d分别给大鼠输注1×1O个/只的供、受体致耐受DC。各组于术后第l、2、4、8周随机处死5只大鼠,进行移植牙病理学检查。结果炎性细胞浸润B组最重,A组最轻,C、D介于二者之间。牙根吸收A组最少,B组最多,C、D介于二者之问(P<0.05);2、4周时牙根吸收C、D组差异无统计学意义(P>0.05),在8周时D组低于C组(P3、高(P<0.05);替代吸收A组最低(P<0.05)。结论两种不同来源的致耐受DC都可以降低牙移植的排斥反应,减少牙根的吸收,促进牙周膜的愈合;其受体致耐受DC更能减少牙根后期的吸收。关键词:牙再移术;树突细胞;牙周膜;牙根吸收;愈合中图分类号:R782;R392.4文献标识码:A文章编号:1671-8348(2014)26—3461—03Ef~ctofdifferentsourcesoftolerogenicdendriticcellsonrootresorptionandhealingaftertoothtransplanta4、ti0ninratsZouLinhong,ZhangLinlin,WangYurong,ShanChuncheng,MingZhiqiang。,ChenYunjia。,LiWenying(1.DepartmentofStomatology,AffiliatedYongchuanHospital,ChongqingMedicalUniversity,Yongchuan,Chongqing402160,China2.DepartmentofOrthodontics,A{ftliatedStomatologicalHospital,Ch5、ongqingMedicalUniversity,Chongqing400015,China)Abstract:0bjectiveTostudytheeffectsofdifferentsourcesofdendriticcells(DCs)onrootresorptionandhealingafterallo—geneictoothtransplantationinrats~MethodsTheBNratsandLewisratswererespectivelyusedasthedonorandrecipientteethfor6、establishingtheanimalexperimentaltransplantationmodelsandrandomlydividedinto4groups,2Ocasesineachgroup:thesyn—geneictransplantationgroup(A)andtheallogeneictransplantationgroups(B,C,D).ThegroupAandBwereinfusedwithPBSbuffersolution0.5mLon7dbeforeoperation.ThegroupCandDw7、ereinfusedwith1×10。donororrecipienttolerogenicDCson7dbeforeoperation.5ratsrandomlyselectedfromeachgroupweresacrificedforperformingthepathologicalexaminationsoftransplantedteethattheendof1,2,4and8weeks.ResultsTheinflammatorycellularinfiltrationwasmostsevereinthegroupB,8、slightestinthegroupAandmoderateinthegroupCandD.TherootresorptionwasminimalinthegroupA,maximalinthegroupBandmoderateinthegrou
3、高(P<0.05);替代吸收A组最低(P<0.05)。结论两种不同来源的致耐受DC都可以降低牙移植的排斥反应,减少牙根的吸收,促进牙周膜的愈合;其受体致耐受DC更能减少牙根后期的吸收。关键词:牙再移术;树突细胞;牙周膜;牙根吸收;愈合中图分类号:R782;R392.4文献标识码:A文章编号:1671-8348(2014)26—3461—03Ef~ctofdifferentsourcesoftolerogenicdendriticcellsonrootresorptionandhealingaftertoothtransplanta
4、ti0ninratsZouLinhong,ZhangLinlin,WangYurong,ShanChuncheng,MingZhiqiang。,ChenYunjia。,LiWenying(1.DepartmentofStomatology,AffiliatedYongchuanHospital,ChongqingMedicalUniversity,Yongchuan,Chongqing402160,China2.DepartmentofOrthodontics,A{ftliatedStomatologicalHospital,Ch
5、ongqingMedicalUniversity,Chongqing400015,China)Abstract:0bjectiveTostudytheeffectsofdifferentsourcesofdendriticcells(DCs)onrootresorptionandhealingafterallo—geneictoothtransplantationinrats~MethodsTheBNratsandLewisratswererespectivelyusedasthedonorandrecipientteethfor
6、establishingtheanimalexperimentaltransplantationmodelsandrandomlydividedinto4groups,2Ocasesineachgroup:thesyn—geneictransplantationgroup(A)andtheallogeneictransplantationgroups(B,C,D).ThegroupAandBwereinfusedwithPBSbuffersolution0.5mLon7dbeforeoperation.ThegroupCandDw
7、ereinfusedwith1×10。donororrecipienttolerogenicDCson7dbeforeoperation.5ratsrandomlyselectedfromeachgroupweresacrificedforperformingthepathologicalexaminationsoftransplantedteethattheendof1,2,4and8weeks.ResultsTheinflammatorycellularinfiltrationwasmostsevereinthegroupB,
8、slightestinthegroupAandmoderateinthegroupCandD.TherootresorptionwasminimalinthegroupA,maximalinthegroupBandmoderateinthegrou
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