不同干预措施对哮喘大鼠免疫耐受的影响

《不同干预措施对哮喘大鼠免疫耐受的影响》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、英文摘要ratsweresensitizedwithintraperitonealinjectionofovalbuminandAL(OH3)，andrepeatedlyexposedtoaerosolizedovalbumin．RatsingroupDreceivedDEX(O．5mg／kg)inperitonealcavity，30rainpriorOVAchallenge．RatsingroupSreceivedOVA(100mg，200mg，400mg)intailhead，discernin33days，35days，

2、37days．Airwayresponsivenessoftheanimalsinfivegroupsweremeasuredandthechangeofairwayhistologywereobservedbyanimallungfunctionmeter．Thecellsinbronchoalveolarlavagefluid(BALF)werecountedandclassified．TheconcentrationsofIFN—Y，IL一4andOVAslgEinBALFandbloodserumweremeasured

3、bysandwichenzyme—linkedimmunosorbentassay(ELISA)．TheairwayinflammationwereobservedwithHEstainingmethod．TheexpressionofGATA-3proteinandT-betproteininpulmonarytissueweredetectedbymeansofimmunohistochemicalstaining．TheexpressionofCD4十CD25+FoxP3十Tcellsweredetectedbyflowc

4、ytometry．Results：1BALFcelelularcomponentThepercentageoftotalcellsandeosinophils(EOS)inBALFofgroupNSwere[(41．67士4．14)X105、(2．67+0．98)×10)]respectively,whichweresignificantlylowerthanthoseingroupA[(124．83+6．45)×105、(33．08士3．50)x105】，groupD[(69．3315．01)xl05(6．4210．90)×1

5、05]，groupS[(76．08士4．29)x105、(6．50士0．79)x105】，groupDS【(67．58士5．63)x105、(5．92士1．24)×103】(尸

6、njectionofacetylcholinechloride(6．25pg／kg，12．599／kg，25pg／kg，50pg／kg)intheasthmaticgroupwas(12．50+0．62)、(37．40+0．68)、(65．95+2．37)、(91．11+0．61)，respectively，(12．52士1．48)、(21．41+1．53)、(36．62+3．67)、(52．64士0．41)ingroupNS，(13．14士0．14)、(31．63+0．91)、(60。95+2．37)、(66．90+0．50)

7、ingroupD，(13．534-0．35)、(32．24土0．78)、(62．03+1．84)、(77．45士1．39)ingroupS，(13．47+0．32)、(29．50+1．23)、(56．47+1．35)、(66．53+0．99)ingroupDS．Resultsofpulmonaryfunctionobservation：thebasicresistanceofexpirationwashigherinasthmagroupthanthoseofthecontrolandtreatedgroup(P<0．05)．T

8、heresistanceofexpirationwashigherinasthmagroupthanthoseofcontrolgroupafterinjectAch(尸