临床医学毕业论文大鼠慢性高眼压视网膜nogoa的表达.doc

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1、毕业论文大鼠慢性高眼压视网膜NowA的表达名：2014年6月25日大鼠慢性高眼压视网膜NogoA的表达作者：聂庆珠，刘致力，沙倩，高殿文【摘要】目的：研究慢性高眼压大鼠视网膜髓鞘相关抑制蛋ANogoA表达的变化。方法：成年雄性Wistar大鼠36只随机分为正常对照组6只和慢性高眼压组30只，应用免疫组织化学方法观察慢性高眼压大鼠视网膜不同时点NogoA表达的变化。结果：与正常对照组相比，慢性高眼压21d大鼠视网膜变薄，节细胞数量减少(P0.05)；NogoA表达增多，与形态学变化相一致(P0.05)o结论：髓鞘相关抑制蛋ANogoA在慢性高眼

2、压大鼠视网膜损伤过程中发挥了重要作用。【关键词】视网膜；慢性高眼压；NogoA；视网膜神经节细胞AbstractAIM:TostudytheexpressivevariationofNogoAonratretinaintheprocessofchronicocularhypcrtcnsion.METHODS:ThirtysixhealthyadultmaleWistarswererandomlydividedintocontrolgroup(6rats)andchronichypertensiongroup(30rats).Chronichy

3、pertensionwascreatedbycauterizingthesuperficialscleralveins.ImmunohistochemistrytechniquewasusedtoevaluatetheexpressivevarietiesofNogoAatdifferenttimepointsduringthecourseofchronicocularhypertension.RESULTS:Thesuccessofthemodelwasindicatedbyover40%ofincreaseintheIOPascompar

4、edwithnormalrats.Comparedwithcontrolgroup,astimepassedchronichypertensiongroupgraduallyhaddetectablemorphologychangesintheretina.Atthe21stdayofchronicocularhypertension,retinasbecamethinnerandthequantityofretinalganglioncell(RGC)decreased(P0.05).Assoicatedwiththemorphologic

5、alchanges,theexpressionofNogoAwasstronglyincreased(P0.05).CONCLUSION:MyelinassociatedproteinNogoAplaysapartintheprocessofchronicocularhypertension.KEYWORDS:retina;chronichypertension;NogoA;retinalganglioncellINTRODUCTIONThepathologicalmechanismofglaucomatousopticneuropathyi

6、sprogressivedeathofretinalganglioncells,whichleadstoirreversibledamage.Regenerationofdamagedcentralnervoussystem,includingopticnerve,isdifficulttoachievebecauseofanumberofreasons,suchasinhibitorsofaxonalregenerationarcpresentinmyelin,lackofneurotrophicfactors,andformationof

7、thegliasea匚IthasbeenpostulatedthattheregenerationofcentralnervoussystemisaffectedbymyelinassociatedproteinNogoA.NogoA,whichispredominantlypresentinoligodendrocytesandmyelinintheadultcentralnervoussystem(CNS),notonlyrestrictsneuritegrowth,plasticity,andaxonalregeneration,but

8、alsolimitstheinvasionandmigrationofcellsandtumorsintheCNS[1].Therefore,ourfindingo