临床医学毕业论文大鼠慢性高眼压视网膜nogoa的表达

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1、大鼠慢性高眼压视网膜NogoA的表达姓名：2014年6月25日作者：聂庆珠，刘致力，沙倩，高殿文【摘要】目的：研究慢性高眼压大鼠视网膜髓鞘相关抑制蛋白NogoA表达的变化。方法：成年雄性Wistar大鼠36只随机分为正常对照组6只和慢性高眼压组30只，应用免疫组织化学方法观察慢性高眼压大鼠视网膜不同时点NogoA表达的变化。结果：与正常对照组相比，慢性高眼压21d大鼠视网膜变薄，节细胞数量减少(P0.05);NogoA表达增多，与形态学变化相一敛(P0.05)。结论：髓鞘相关抑制蛋白NogoA在慢性高眼压火鼠视网膜损伤过程屮发挥了重

2、要作用。【关键词】视网膜；慢性高眼压；NogoA；视网膜神经节细胞AbstractAIM:TostudytheexpressivevariationofNogoAonratretinaintheprocessofchronicocularhypertension.METHODS:ThirtysixhealthyadultmaleWistarswererandomlydividedintocontrolgroup(6rats)andchronichypertensiongroup(30rats).Chronichypertension

3、wascreatedbycauterizingthesuperficialscleralveins.ImmunohistochemistrytechniquewasusedtoevaluatetheexpressivevarietiesofNogoAatdifferenttimepointsduringthecourseofchronicocularhypertension.RESULTS:Thesuccessofthemodelwasindicatedbyover40%ofincreaseintheIOPascomparedwit

4、hnormalrats.Comparedwithcontrolgroup，astimepassedchronichypertensiongroupgraduallyhaddetectablemorphologychangesintheretina.Atthe21stdayofchronicocularhypertension，retinasbecamethinnerandthequantityofretinalganglioncell(RGC)decreased(P0.05).Assoicatedwiththemorphologic

5、alchanges，theexpressionofNogoAwasstronglyincreased(P0.05).CONCLUSION:MyelinassociatedproteinNogoAplaysapartintheprocessofchronicocularhypertension.KEYWORDS:retina;chronichypertension;NogoA;retinalganglioncellINTRODUCTIONThepathologicalmechanismofglaucomatousopticneurop

6、athyisprogressivedeathofretinalganglioncells，whichleadstoirreversibledamage.Regenerationofdamagedcentralnervoussystem,includingopticnerve，isdifficulttoachievebecauseofanumberofreasons,suchasinhibitorsofaxonalregenerationarepresentinmyelin，lackofneurotrophicfactors，andf

7、ormationofthegliascar.IthasbeenpostulatedthattheregenerationofcentralnervoussystemisaffectedbymyelinassociatedproteinNogoA.NogoA，whichispredominantlypresentinoligodendrocytesandmyelinintheadultcentralnervoussystem(CNS)，notonlyrestrictsneuritegrowth,plasticity,andaxonal

8、regeneration，butalsolimitstheinvasionandmigrationofcellsandtumorsintheCNS[1].Therefore，ourfindingontheexpressivevaria