替普瑞酮对大鼠慢性高眼压视网膜hsp70表达的影响

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1、替普瑞酮对大鼠慢性高眼压视网膜HSP70表达的影响：刘致力，王英蓉，沙倩，聂庆珠【摘要】　　目的：研究慢性高眼压大鼠视网膜HSP70表达的变化及替普瑞酮的影响。方法：:Tostudytheeffectsofgeranylgeranylacetone(GGA)ontheexpressionofheatshockprotein70(HSP70)onretinalganglioncells(RGC)inratsg/(kg·d)GGAmunohistochemistryodelratsandGGAinterferenceratsatdifferenttimepointsduringthecours

2、eofchronicIOPelevation.RESULTS:Thesuccessfulmodelalrats.Theretinalthicknessodelgroupandmodel+GGAgroupparedalratsfrom21daysthrough28daysaftercauterization(P<0.05),andthatofmodelratsodel+GGArats(P<0.05).Thenumberofganglioncellsodelratsandmodel+GGAratsparedalratsfrom21daysand28days.Thestrongere

3、xpressionintensity(IOD)valueodel+GGAratsbyimmunochemistry(P<0.01).CONCLUSION:SystemicadministrationofGGAprotectsretinafromchronicIOPelevationbyregulatingtheexpressionofHSP70.　　KEYATERIALSANDMETHODS　　MaterialsSeventymaleedicalUniversity.TheanimalsandexperimentalconditionsfolloalregulationsofStat

4、eScienceandTechnologymission.Theanimalslydividedinto3groups,odelofchronicIOPelevation.TheratsL/kg,bulbarconjunctivabus);bulbarconjunctivaeasuredeasurementtimepointsmHg)meansthemodelingodelingg/(kg·d).Themodelingproceduree.　　ImmunohistochemistryAftersamplingfixation,dehydrationandparaffinimbeddinge

5、daccordingtotheinstructionofthekit.Positivecellsornuclei..MeandataamonggroupsodelingtheretinabecamethinnerandthenumberofRGCdecreased,hoedicationgroupild.AmongthreegroupsatleastthaddistinctivedifferenceaccordingtotheanalysisbyLevenehomogeneitytestforvariance.Intergroupmultipleparisontestindicatedt

6、hat,intermsofthicknessparison,paredodelgroupandmodel+GGAgrouphadstatisticalsignificance(P<0.05,Table1).　　HSP70ExpressionItisillustratedinFigure1.Controlgrouperelydetectedinlayerofganglioncellsintheretinaofnormalrats.HSP70munecellsforHSP70seemedtobereducedinretinaofhighIOPfor7daysgroup.Inocularh

7、ypertensionfor21daysgroup,theexpressionofHSP70inretinaberofroundsyncytiumbigpositivecellsforHSP70inganglioncelllayerbegantoincrease.HSP70ayinterfereultiplestagesoftheapoptoticpathinalkinase(JNK)activation,prevent