大鼠慢性高眼压视网膜nogoa的表达

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1、大鼠慢性高眼压视网膜NogoA的表达作者：聂庆珠，刘致力，沙倩，高殿文【摘要】　　目的：研究慢性高眼压大鼠视网膜髓鞘相关抑制蛋白NogoA表达的变化。方法：成年雄性Wistar大鼠36只随机分为正常对照组6只和慢性高眼压组30只，应用免疫组织化学方法观察慢性高眼压大鼠视网膜不同时点NogoA表达的变化。结果：与正常对照组相比，慢性高眼压21d大鼠视网膜变薄，节细胞数量减少(P<0.05)；NogoA表达增多，与形态学变化相一致(P<0.05)。结论：髓鞘相关抑制蛋白NogoA在慢性高眼压大鼠视网膜损伤过程中发挥了重要作用。【

2、关键词】视网膜；慢性高眼压；NogoA；视网膜神经节细胞　　AbstractAIM:TostudytheexpressivevariationofNogoAonratretinaintheprocessofchronicocularhypertension.METHODS:ThirtysixhealthyadultmaleWistarswererandomlydividedintocontrolgroup(6rats)andchronichypertensiongroup(30rats).Chronichypertensionwascre

3、atedbycauterizingthesuperficialscleralveins.8ImmunohistochemistrytechniquewasusedtoevaluatetheexpressivevarietiesofNogoAatdifferenttimepointsduringthecourseofchronicocularhypertension.RESULTS:Thesuccessofthemodelwasindicatedbyover40%ofincreaseintheIOPascomparedwithnormalrats

4、.Comparedwithcontrolgroup,astimepassedchronichypertensiongroupgraduallyhaddetectablemorphologychangesintheretina.Atthe21stdayofchronicocularhypertension,retinasbecamethinnerandthequantityofretinalganglioncell(RGC)decreased(P<0.05).Assoicatedwiththemorphologicalchanges,thee

5、xpressionofNogoAwasstronglyincreased(P<0.05).CONCLUSION:MyelinassociatedproteinNogoAplaysapartintheprocessofchronicocularhypertension.　　KEYWORDS:retina;chronichypertension;NogoA;retinalganglioncell　　INTRODUCTION　　Thepathologicalmechanismofglaucomatousopticneuropathyispr

6、ogressivedeathofretinalganglioncells,whichleadstoirreversibledamage.Regenerationofdamagedcentral8nervoussystem,includingopticnerve,isdifficulttoachievebecauseofanumberofreasons,suchasinhibitorsofaxonalregenerationarepresentinmyelin,lackofneurotrophicfactors,andformationoftheg

7、liascar.IthasbeenpostulatedthattheregenerationofcentralnervoussystemisaffectedbymyelinassociatedproteinNogoA.NogoA,whichispredominantlypresentinoligodendrocytesandmyelinintheadultcentralnervoussystem(CNS),notonlyrestrictsneuritegrowth,plasticity,andaxonalregeneration,butals

8、olimitstheinvasionandmigrationofcellsandtumorsintheCNS[1].Therefore,