大鼠慢性高眼压视网膜nogoa的表达

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1、大鼠慢性高眼压视网膜NogoA的表达【关键词】视网膜；慢性高眼压；NogoA；视网膜神经节细胞　　AbstractAIM:TostudytheexpressivevariationofNogoAonratretinaintheprocessofchronicocularhypertension.METHODS:ThirtysixhealthyadultmaleedicalUniversity.TheanimalsandexperimentalconditionsedinaccordancealregulationsofStateScienceandTechnolog

2、ymission.Animalslydividedinto2groups,odelofchronicIOPelevation.RatsL/kgofchloralhydrate(100g/L).Bulbarconjunctivabus.BulbarconjunctivaeasuredmHg)indicatesasuccessofthemodel.　　Aftersamplingfixation,dehydrationandparaffinimbeddingedaccordingtotheinstructionofthekit.Positivecellsornuclei.orph/

3、BX51microgramanalyticalsystemtodeterminetheintegratedAofpositivecells.　　StatisticalAnalysisQuantitativedataean±SEM,andultipleparisonsamongexperimentalgroupsandcontrolgroups.StatisticalanalysisedusingtheSPSS17.0statisticalsoftware.TheresultswereconsideredstatisticallydifferentatP<0.05.　　R

4、ESULTS　　After21daysofchronicocularhypertensiontheretinabecamethinner(28.3±8.0,17.0±6.5μm,P<0.05)andthenumberofRGCdecreasedasparedountofNogoAratmodel,P<0.01)afterestablishmentofthemodel,andtheincreaseremainedsignificantlyat28days(37.69±3.15,24.93±1.31μm,P<0.05)afterthemodelestablis

5、hmentparedageofglauaisachroniccourse.Hoostanimalmodelsforglauaresearchareischemiareperfusionmodelsandhavedisadvantageforobservationofretinaprotection.Reportsaboutmorphologicalchangesunderchronicocularhypertensionarerare.RegenerativenervefibergroitedintheSofadultmammalian,includingopticnerv

6、e,inpartbecauseofthepresenceofneuritegroportantstepinelucidatingthemechanismsofthisinhibitionajorproteinproducts,NogoA,B,andC,byalternativesplicingandalternativepromoterusage[6,7].NogoAaticallyincreasedNogoAindicatedthatNogoAmayplayanimportantroleinobstructingregenerationofopticnerve.

7、SuppressionoftheNogoAmightbeaneentforglaua.【参考文献】　　1BelienATJ,PagatiPA,Schbranetype1matrixmetalloprotease(MT1MMP)enablesinvasivemigrationofgliomacellsincentralnervoussystematter.JCellBiol1999;144:373383　　2BeharO,MizunoK,NeumannS,S,HuberAB,vanderHaarM