建立HILIC-UPLC联用分析法测定血浆中葫芦巴碱及在药动学研究中的应用-论文.pdf

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1、Availableonlineatwww．sciencedirect．comChinese端ScienceDirectJournalofNaturalMedicinesChineseJournalofNaturalMedicines2013，l1(2)：0164—0170doi：10．3724／SEJ．1009-2013．00164Development0fahydrophilicinteractionchromatography-—UPLCassaytodeterminetrigonellineinrat’

2、1‘l●‘●’l■·■'OlasmaannitsapplicationinapharmacoKineticstudyCHENGZai—Xing1，Jin—Jun0，LIUZhong．Qiu1}，LINNa1Co／legeofPharmacy,FujianUniversityofTraditionalChineseMedicine,Fuzhou350108．China,"。DepartmentofPharmaceutics,SchoolofPharmaceuticalSciences,SouthernMedic

3、alUniversit~Guangzhou510515,China,SchoolofChineseMateriaMedica，GuangzhouUniversityofChineseMedicine,Guangzhou510006,China,InstituteofChineseMeteriaMedica,ChinaAcademyofChineseMedicalSciences,Beo'ing100700,ChinaAvailableonline20Mar．2013【ABSTRACTIAIM：Trigonel

4、line(Tr)isthesecondmostabundantalkaloidincofeebeans．Thisstudydevelopedanassaycombininghydrophilicinteractionchromatographywithultraperformanceliquidchromatography(HILIC—UPLC)forthequantificationofTrinratplasmatodetermineitspharmacokineticbehavior．METHODS：Af

5、tertheadministrationofTrbygavageaswelIasintravenousiniectionandthatofmethanolextractofcofleebeans(MECB1orally．bloodsamplesfromtheexperimentalratswereanalyzedusingtheHILIC—UPLCassay．Pharmacokineticparametersweredeterminedusingthestandardnon—compartmentalmeth

6、odandcalculatedusingPracticalPharmacokineticProgramVersion87／97．RESULTS：TheHILIC—UPLCassaywasvalidatedwiththe1inearrangeof0．12—100g-mL～andalowerlimitofquantitationof0．12lag‘mL～．Itsaccuracy,precision，recovery，andstabilitywerewithinacceptable1imits．TheAUCr0-、

7、(whereAUCistheareaundertheplasmaconcentration-timecurve)valuesweredeterminedtobe(4066．83±1244．41)and(3544．29±908．80)min‘g·mL～afterTrwasorallyandintravenouslyadministered，respectively．Itwasf4566．75±1435．64)min·ug·mL～afterMECBwasorallyadministered．Theabsolute

8、bioavailabilityofTralonereached57．37％，whereasthatofTrinMECBwas64．42％．Therelativebioavailabilityofthealkaloidwas112-29％．C0NCLUSIoNS：TheHILIC．UPLCassayforTrdeterminationissimpleandaccurate，andalsoexhibit