两种玻璃化冷冻方法在大鼠卵巢组织冷冻中的应用效果观察-论文.pdf

《两种玻璃化冷冻方法在大鼠卵巢组织冷冻中的应用效果观察-论文.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、解放军医药杂志2015年5月第2Z卷第!!垒!：：型：·9··论著·两种玻璃化冷冻方法在大鼠卵巢组织冷冻中的应用效果观察李冬秀，耿杨柳，吴小华，于晓惠，张敏，徐清华，许鹏宇[摘要]目的比较直接覆盖玻璃化冷冻(directcovervitrification，DCV)及常规玻璃化冷冻(conventionalvitrifi-cation，cv)在大鼠卵巢组织冷冻中的应用效果。方法将雌性Wistar大鼠30只随机分为A、B、C组，每组1O只，切除大鼠双侧卵巢组织。A组为对照组，B组和c组分别应用CV和DCV冻存2周。复苏后观察3组卵泡的形态，并用末端脱氧核酰转移酶介导的脱氧尿苷三磷酸

2、标记实验(TUNEL)检测各组卵泡的凋亡率。结果A组各级卵泡形态正常；B组卵泡形状不规则，卵母细胞核固缩；c组较大的窦状卵泡损伤。除C组原始卵泡外，B组和c组形态正常的各级卵泡比例均低于A组(P<0．05)；C组形态正常的各级卵泡比例高于B组(P<0．05)。B、c组卯泡凋亡率高于A组，且c组低于B组(P<0．01)。结论DCV较CV卵巢组织效果更好，可以有效保存卵巢组织中的原始卵泡。[关键词】卵巢；玻璃化作用；低温保存；大鼠，Wistar[中国图书资料分类号]R715；R一332[文献标志码]A[文章编号]2095—140X(2015)05—0009-04[DOI]10．396

3、9／j．issn．2095—140X．2015．05．003ComparisonofEffectsofTWOKindsofVitrificationMethodsinFreezing0varianTissuesinRatsLIDong—xiu，GENGYang—liu，wuXiao·hua，YuXiao—hui，ZHANGMin，XUQing—hua，XUPeng—yu(CenterofRe—productiveMedicineCenter，BethuneInternationalPeaceHospitalofPLA，Shijiazhuang050082，China)[Abstr

4、act]0bjectiveTocomparetheeffectsofdirectcovervitrification(DCV)andconventionalvitrification(CV)infreezingovariantissuesinrats．MethodsAtotalof30maturefemaleWistarratswererandomlydividedintogroupA，BandC(n=10foreachgroup)，andbilateralovariantissuesofallratswerecutof．GroupAwasthecontrolgroup；grou

5、pBandCwererespectivelyfreezedusingCVorDCVmethodfor2weeks．Afterthawed，inthethreegroups，thefollicularappearanceswereobserved，andfollicularapoptosisratesweredetectedusingTUNEL(terminaldeoxynucle—otidyltransferase-mediateddUTPnickendlabeling)method．ResultsTheappearancesofeachgradewerenormalingrou

6、pA；thefollicularappearanceswereirregularandoocytekaryopyknosiswasfoundingroupB；thebiggersinusoidO—varianfolliclesweredamagedingroupC．ThenormalratesoffollicularappearancesinallgradesofgroupBandCweresignificantlylowerthanthoseingroupAexceptforthatofprimordialfolliclesingroupC(P<0．05)；thenormalr

7、atiosoffollicularappearancesinallgradesofgroupBweresignificantlyhigherthanthoseingroupB(P<0．05)．Thefollicu—larapoptosisratesingroupBandCweresignificantlyhigherthanthatingroupA，andtherateingroupCwassignifi—cantlylowerthanthatingroupB(P<0．01)．C