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1、·412·中国实验血液学杂志JournalofExperimentalHematology2005;13(3):412-416文章编号(ArticleID):1009-2137(2005)03-0412-05·论著·树突状细胞介导的抗白血病效应的临床研究陈虎,楼晓,江岷,胡亮钉,俞志勇,徐晨,李渤涛,宁红梅,李欲航,冯凯,刘广贤军事医学科学院附属医院造血干细胞移植科,北京100039摘要肿瘤的免疫治疗作为恶性肿瘤综合治疗的重要组成部分,近年来受到了广泛的关注。树突状细胞(DC)是已知的功能最强的抗原呈递细胞,不
2、仅能够激活自体的抗肿瘤免疫,同样能够提高异体免疫组织的抗肿瘤效应。为了探讨外周血来源的DC体外培养扩增和临床治疗的可行性和安全性,分析DC免疫治疗对于急性非淋巴细胞白血病自体骨髓移植患者长期生存的影响,利用CS3000Plus采集13例急性非淋巴细胞白血病自体骨髓移植后的病人的外周血单个核细胞,经过2周的体外培养后行DC免疫治疗,治疗后长期随访观察其无病生存时间。结果表明,无一例发现有与DC免疫治疗相关的严重不良反应;Kaplan2Meier生存分析结果为:DC组5年生存率为75152%,非DC组5年生存率为4
3、5.71%,DC组在累计生存率上明显优于非DC组。结论:外周血来源的DC体外培养和临床治疗是安全可行的,急性非淋巴细胞白血病病人在自体骨髓移植术后应用DC免疫治疗延长了其无病生存时间,提高了长期生存率。关键词树突状细胞;抗白血病效应;自体骨髓移植;免疫治疗;细胞治疗中图分类号R730.51;R457.7文献标识码AClinicalStudyonAnti2leukemiaEffectMediatedbyDentriticCellsCHENHu,LOUXiao,JIANGMin,HULiang2Ding,YUZhi
4、2Yong,XUChen,LIBo2Tao,NINGHong2Mei,LIYu2Hang,FENGKai,LIUGuang2XianDepartmentofHematopoieticStemCellTransplantation,TheAffiliatedHospitalofAcademyofMilitaryMedicalSciences,Beijing100039,ChinaAbstractImmunotherapyoftumorisextensivelyattentionedasanimportantpar
5、tofcombinedtherapyoftumorinrecentyears.Dendriticcell(DC)isthemostpowerfulantigenpresentingcell(APC)bynowwhichnotonlyactivatesauto2immunitytoattacktumorcells,butalsodoeshelptoenhanceantitumoreffectforallogenicbodies.Toexplorethefeasibilityandsafetyofclinicalt
6、herapyapplicationofperipheralbloodderivedDCculturedexvivo,andanalyzetheinfluenceofDC2inducing2immunotherapyuponlong2termsurvivalofANLLpatientsacceptedautologousbonemarrowtransplantation,peripheralbloodmononuclearcells(PBMNC)of13ANLLpatientsafterautologousbon
7、emarrowtransplantationwerecollectedbyusingCS3000Plus.DCimmunotherapywasadministeredaftercultivationofPBMNCexvivofor2weeks,desease2freesurvivaltimewasobservedaftertherapyforlongtimefollow2up.TheresultsshowedthatnoanysevereadverseeventassociatedwithDCtherapywa
8、sobserved,thesurvivalanalysisofKaplan2Meiersuggestedthatfiveyearsurvivalratewas75.52%inDCgroupwhile45.71%innon2DCgroup.DCgroupsurpassednon2DCgroupinaccumulativesurvivalrate.Itisconcludedthatthee
