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1、体外消化对蓝莓提取物抗氧化、抗癌及组分的影响薛宏坤1，谭佳琪2，王瑜3，王鑫3,4（1.东北农业大学工程学院，黑龙江哈尔滨150030；2.东北农业大学农学院，黑龙江哈尔滨150030；3.东北农业大学食品学院，黑龙江哈尔滨150030；4.哈尔滨海关，黑龙江哈尔滨150028）摘要：以蓝莓为原料，考察了体外消化前、后蓝莓提取物（总酚和花色苷）含量、抗氧化性、抗癌作用及花色苷组成的变化，并推测了花色苷的降解途径。分别采用福林-酚法和pH示差法测定总酚和花色苷含量；以脂质体的抑制率、DPPH和ABTS+自由基清除能力评价体外消化前后提取物的抗氧化能力；利用高效液相色谱-电喷雾二级质谱联用技术分
2、析花色苷组成。结果表明：与未经消化提取物相比，经肠道消化后样品总酚含量增加47.21%，花色苷含量降低71.82%，脂质体的抑制率、DPPH和ABTS+自由基清除率分别提高38.45%、29.41%和29.12%；对HepG2肝癌细胞、A549肺癌细胞和Hela人宫颈癌细胞的生长抑制作用显著增加，蓝莓花色苷组分由12种降为9种。对胃肠消化花色苷的降解过程推测发现，矢车菊素-3-葡萄糖苷降解形成槲皮素的过程与天竺葵素-3-葡萄糖苷降解成山柰酚的过程机制相同。关键词：体外消化；蓝莓提取物；抗氧化性；抗癌作用；花色苷组分中图分类号：TS255.42文献标志码：A文章编号：Effectsofinvi
3、troDigestiononAntioxidantActivity,Anti-cancerActivityandCompositionofBlueberryExtractsXUEHong-kun1,TANJia-qi2,WANGYu3,WANGXin3,4(1.CollegeofEngineering,NortheastAgriculturalUniversity,Harbin150030,Heilongjiang,China;2.CollegeofAgriculture,NortheastAgriculturalUniversity,Harbin150030,Heilongjiang,Ch
4、ina;3.CollegeofFood,NortheastAgriculturalUniversity,Harbin150030,Heilongjiang,China;4.HarbinCustoms,Harbin150028,Heilongjiang,China)Abstract:Changesintotalpolyphenolsandanthocyaninscontents,antioxidantcapacityandanticancereffect,anthocyaninscompositionsofblueberryextractsbeforeandafterinvitrodigest
5、ionwereinvestigated.Thedegradationpathwayofanthocyaninswasspeculated.ThetotalpolyphenolscontentandanthocyaninscontentweremeasuredbyFolin-CiocalteureagentmethodandpHdifferentialmethod,respectively.Theantioxidantcapacityoftheextractsbeforeandafterinvitrodigestionwasevaluatedbytheinhibitionrateoflipos
6、omes,1-diphenyl-2-picrylhydrazyl(DPPH)andABTS+freeradicalsscavengingabilities.Furthermore,thecompositionsofanthocyaninswereanalyzedusingbyhighperformanceliquidchromatography-electrosprayionizationmassspectrometry(HPLC-EIS-MS).Theresultsindicatedthatafterinvitrodigestionthecontentoftotalpolyphenolsi
7、ncreasedby47.21%,whilethatofanthocyaninsdecreasedby71.82%,andtheinhibitionrateofliposomes,scavengingratesofDPPHandABTS+freeradicalsincreasedby38.45%,29.41%and29.12%,respectively.Blueberryextractscouldsignif
