仙茅提取物体外抗氧化活性研究

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1、仙茅提取物体外抗氧化活性研究作者：张振东吴兰芳景永帅杨娟【摘要】目的探讨仙茅提取物的抗氧化活性。方法先用80%乙醇，然后用蒸馏水对仙茅进行提取，其中醇提浸膏依次用石油醚、氯仿、乙酸乙酯、正丁醇萃取；水提部分经醇沉，制备仙茅粗多糖。分别观察仙茅各提取物对羟基自由基（·OH）、1,1二苯基2苦基苯肼自由基(DPPH·)的清除率以及对Fe3+还原力。结果与抗坏血酸比较，仙茅提取物对自由基均有较强清除作用，对·OH的清除率乙酸乙酯萃取物＞正丁醇萃取物＞粗多糖＞总醇提物，IC50值分别为0.247、0.358、0.796和0.871mg

2、/ml；对DPPH·的清除率乙酸乙酯萃取物>正丁醇萃取物>总醇萃取物>氯仿萃取物>石油醚萃取物>粗多糖，其IC50值分别为0.026、0.055、0.620、0.071、0.122和0.275mg/ml。不同提取物对Fe3+有较强还原能力，还原力大小为：乙酸乙酯萃取物>正丁醇萃取物>总醇提物>氯仿萃取物>石油醚萃取物>粗多糖。结论仙茅具有明显的抗氧化活性，在抗衰老方面有广泛的应用前景。【关键词】仙茅；抗氧化能力；清除自由基；还原力【Abstract】ObjectiveTos

3、tudyonantioxidantactivitiesofextractsfromCurculigoorchioideinvitro.MethodsCurculigo11orchioidesweretreatedwith80%ethanolandthentheresiduewereextractedwithwater.Theethanolextractwereextractedrespectivelyusingpetroleumether,chloroform,ethylacetate,nbutanol.Atthesametime

4、,crudepolysaccharidewasobtainedbyethanolprecipitationfromwaterextract.Hydroxylfreeradical（·OH）,DPPHfreeradical(DPPH·)anddeoxidizationFe3+reactionsystemwereusedtoobserveinvitroantioxidanteffectsoftheaboveextracts.ResultsComparedwiththeascorbicacid,theextractshadstrongsc

5、avengingeffectonfreeradicals,theactivitiessequenceforscavenging·OHwereethylacetateextract>nbutanolextract>polysaccharide>ethanoltotalextract,whichcorrespondingIC50valueswere0.247,0.358,0.796and0.871,respectively.ThecapabilitiesofscavengingDPPHwereethylacetate

6、extract>nbutanolextract>ethanoltotalextract>chloroformextract>petroleumetherextract>polysaccharide,andthecorrespondingIC50valueswere0.026,0.055,0.620,0.071,0.122and0.275mg/ml,respectively.DifferentextractshadstrongdeoxidizationabilityofFe3+,theactivitie

7、ssequencewereethylacetateextract>nbutanolextract>ethanoltotalextract>chloroformextract>petroleumetherextract>polysaccharide.ConclusionsCurculigoorchioideshavestronger11antioxidativeactivitiesandwillbewidelyapplicatedonantiaging.　　【Keywords】Curculigoorch

8、ioides;Antioxidantactivity;Radicalscavenging;Deoxidization　　仙茅为仙茅科仙茅属植物仙茅(CurculigoorchioidesGaertn.)的干燥根茎，其性温，味苦，有小毒