ANNATTO EXTRACT (ALKALI-PROCESSED NORBIXIN)-胭脂树橙提取物(降红木素碱性加工).pdf

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1、ANNATTOEXTRACT(ALKALI-PROCESSEDNORBIXIN)(TENTATIVE)Preparedatthe61stJECFA(2003)andpublishedinFNP52Add11(2003).Thepreviousspecificationsforannattoextracts(oilandalkali-extracted)preparedatthe46thJECFA(1996),publishedinFNP52Add4(1996)havebeenreplacedbytheseandseparatespecificationsfor“

2、Annattoextract(aqueous-processedbixin)”,“Annattoextract(alkali-processednorbixin,notacid-precipitated)”and“Annattoextract(oil-processedbixin)”.AtemporaryADIof0–0.4mg/kgbwwasestablishedatstthe61JECFA(2003).Informationrequiredonchemicalcharacterisationofthenon-pigmentcomponentofcommerc

3、ialproductsSYNONYMSL.Orange,CI(1975)75120(NaturalOrange4),INS160bDEFINITIONSeedsfromtheannattotree(BixaorellanaL.)areabradedincoldaqueousalkali(potassiumorsodiumhydroxide).Additionalalkaliisaddedtotheresultingsuspensionwhichisthenheatedtodissolvethepigmentandcooled.Fatsandwaxesarerem

4、oved.Theaqueoussolutionisfilteredandacidifiedtoprecipitatethenorbixin.Theprecipitateisfiltered,washed,driedandmilledtogiveagranularpowder.Alkali-processedannattowillcontainthepotassiumorsodiumsaltofnorbixinasthemajorpigment.Thermaldegradationproductsmayalsobepresentasaresultofprocess

5、ing.Themajorcolouringprincipaliscis-norbixin.Aminorcolouringprincipalistrans-norbixin.Chemicalname9'-cis-6,6'-Diapocarotene-6,6'-dioicacid;mono-ordi-sodiumsalt,mono-ordi-potassiumsaltC.A.S.numbercis-Norbixin626-76-6ChemicalformulaC24H28O4,C24H26Na2O4,C24H26K2O4StructuralformulaR=H,Na

6、,KFormulaweight380.48(acid),424.45(sodiumsalt);456.68(potassiumsalt)AssayNotlessthan35%pigment(expressedasnorbixin)DESCRIPTIONDarkred-browntored-purplepowderFUNCTIONALUSESColourCHARACTERISTICSIDENTIFICATIONSolubility(Vol.4)Solubleinalkalinewater,slightlysolubleinethanolUV/VISabsorpti

7、onThesamplein0.5%potassiumhydroxidesolutionshowsabsorbance(Vol.4)maximaatabout453nmand482nm.ThinLayerActivateaTLCplate(e.g.LK6DSILICAGEL60A(layerthickness:Chromatography250µm,size:5x20cm))for1hat110˚.Preparea5%solutionofthesamplein95%ethanolandapply10µltotheplate.Allowtodryanddevelop

8、usingamixtur