ANNATTO EXTRACT (OIL-PROCESSED BIXIN)-胭脂树橙提取物(红木素石油萃取).pdf

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1、ANNATTOEXTRACT(OIL-PROCESSEDBIXIN)(TENTATIVE)Preparedatthe61stJECFA(2003)andpublishedinFNP52Add11(2003).Thepreviousspecificationsforannattoextracts(oilandalkali-extracted)preparedatthe46thJECFA(1996),publishedinFNP52Add4(1996)havebeenreplacedbytheseandsepa

2、ratespecificationsfor“Annattoextract(alkali-processednorbixin)”,“Annattoextract(alkali-processednorbixin,notacid-precipitated)”and“Annattoextract(aqueous-processednorbixin)”.Duesttothelackoftoxicitydata,noADIwasestablishedatthe61JECFA(2003).Informationrequ

3、iredonchemicalcharacterisationofthenon-pigmentcomponentofcommercialproductsSYNONYMSL.Orange,CI(1975)75120(NaturalOrange4),INS160bDEFINITIONSeedsfromtheannattotree(BixaorellanaL.)areabradedinhotvegetableoiltoremovepigmentfromthesurfaceoftheseeds.Theoilissie

4、vedtoremoveseeds.Themajorcolouringprincipaliscis-bixin.Aminorcolouringprincipalistrans-bixin.Chemicalnames9'-cis-6,6'-Diapocarotene-6,6'-dioicacid,monomethylesterC.A.S.numbercis-Bixin6983-79-5ChemicalformulaC25H30O4StructuralformulaFormulaweight394.51Assay

5、Notlessthan10%pigment(expressedasbixin)DESCRIPTIONDarkred-browntored-purpleoilFUNCTIONALUSESColourCHARACTERISTICSIDENTIFICATIONSolubility(Vol.4)Insolubleinwater,slightlysolubleinethanolUV/VISabsorptionThesampleinacetoneshowsabsorbancemaximaatabout425,457an

6、d(Vol.4)487nmThinLayerChromatographyActivateaTLCplate(e.g.LK6DSILICAGEL60A(layerthickness:250µm,size:5x20cm))for1hat110˚.Preparea5%solutionofthesamplein95%ethanolandapply10µltotheplate.Allowtodryanddevelopusingamixtureofn-butanol,methylethylketoneand10%aqu

7、eousammonia(3:2:2byvolume)untilthesolventfronthasascendedabout10cm.Allowtodry.BixinandnorbixinappearasyellowspotswithRfvaluesofabout0.50to0.45,respectively.Spraywith5%sodiumnitritesolutionandthenwith0.5mol/lsulfuricacidandthespotsimmediatelydecolourise.PUR

8、ITYArsenic(Vol.4)Notmorethan1mg/kgLead(Vol.4)Notmorethan2mg/kgDetermineusinganatomicabsorptiontechniqueappropriatetothespecifiedlevel.Theselectionofthesamplesizeandmethodofsamplepreparationmaybebasedontheprin