ANNATTO EXTRACT (AQUEOUS-PROCESSED BIXIN)-胭脂树橙提取物(红木素水性加工).pdf

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1、ANNATTOEXTRACT(AQUEOUS-PROCESSEDBIXIN)(TENTATIVE)Preparedatthe61stJECFA(2003)andpublishedinFNP52Add11(2003).Thepreviousspecificationsforannattoextracts(oil-andalkali-extracted)preparedatthe46thJECFA(1996),publishedinFNP52Add4(1996)havebeenreplacedbytheseandseparatespe

2、cificationsfor“Annattoextract(alkali-processednorbixin)”,“Annattoextract(alkali-processednorbixin,notacid-precipitated)”and“Annattoextract(oil-processedbixin)”.AtemporarystADIof0–4mg/kgbwwasestablishedatthe61JECFA(2003).Informationrequiredonchemicalcharacterisationoft

3、henon-pigmentcomponentofcommercialproducts;anappropriatemethodtodeterminenorbixinatthespecifiedlimit.SYNONYMSL.Orange,CI(1975)75120(NaturalOrange4),INS160bDEFINITIONSeedsfromtheannattotree(BixaorellanaL.)areabradedincoldaqueousalkali(potassiumorsodiumhydroxide)toremov

4、epigment.Theresultantsuspensionisacidified(sulfuricacid)toprecipitatethebixin.Theprecipitateisfiltered,washeddriedandmilled,togiveagranularpowder.Thermaldegradationproductsmayalsobepresentasaresultofprocessing.Themajorcolouringprincipaliscis-bixin.Aminorcolouringprinc

5、ipalistrans-bixin.Chemicalname9'-cis-6,6'-Diapocarotene-6,6'-dioicacid,monomethylesterC.A.S.numbercis-Bixin6983-79-5ChemicalformulaC25H30O4StructuralformulaFormulaweight394.51AssayNotlessthan25%pigment(expressedasbixin)Pigmentmustcontainnotmorethan7%norbixinDESCRIPTIO

6、NDarkred-browntored-purplepowderFUNCTIONALUSESColourCHARACTERISTICSSolubility(Vol.4)Insolubleinwater,slightlysolubleinethanolUV/VISabsorptionThesampleinacetoneshowsabsorbancemaximaatabout425,457and(Vol.4)487nm.ThinLayerChromatographyActivateaTLCplate(e.g.LK6DSILICAGEL

7、60A(layerthickness:250µm,size:5x20cm))for1hat110˚.Preparea5%solutionofthesamplein95%ethanolandapply10µltotheplate.Allowtodryanddevelopusingamixtureofn-butanol,methylethylketoneand10%aqueousammonia(3:2:2byvolume)untilthesolventfronthasascendedabout10cm.Allowtodry.Bixin

8、andnorbixinappearasyellowspotswithRfvaluesofabout0.50to0.45,respectively.Spraywith5%sodiumnitritesolutionandthenwith0.5mol/l