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1、ANNATTOEXTRACT(SOLVENT-EXTRACTEDBIXIN)(TENTATIVE)Preparedatthe61stJECFA(2003)andpublishedinFNP52Add11(2003).Thepreviousspecificationsforannattoextracts(solvent-extracted)preparedatthe46thJECFA(1996),publishedinFNP52Add4(1996)havebeenreplacedbytheseandseparatespec
2、ificationsfor“Annattoextract(solvent-extractednorbixin)”.AtemporaryADIof0–7mg/kgbwwasestablishedatstthe61JECFA(2003).Informationrequiredonchemicalcharacterisationofthenon-pigmentcomponentofcommercialproducts;anappropriatemethodtodeterminenorbixinatthespecifiedlim
3、itSYNONYMSL.Orange,CI(1975)75120(NaturalOrange4),INS160bDEFINITIONSeedsfromtheannattotree(BixaorellanaL.)areextractedwithsolventtodissolvepigment.Theextractisfilteredtoremoveinsolublematerial.Subsequentprocessinginvolvesremovaloffatsandwaxes,solventremoval,crysta
4、llisationanddrying.Solventsusedcanbeoneormoreof:hexane,acetone,ethanol,(alkaline)methanol,isopropanol,ethylacetate.Thermaldegradationproductsmayalsobepresentasaresultofprocessing.Themajorcolouringprincipaliscis-bixin.Aminorcolouringprincipalistrans-bixin.Chemical
5、name9'-cis-6,6'-Diapocarotene-6,6'-dioicacid,monomethylesterC.A.S.numbercis-Bixin6983-79-5ChemicalformulaC25H30O4StructuralformulaFormulaweight394.51AssayNotlessthan85%pigment(expressedasbixin)Pigmentmustcontainnotmorethan2.5%norbixinDESCRIPTIONDarkred-browntored
6、-purplepowderFUNCTIONALUSESColourCHARACTERISTICSIDENTIFICATIONSolubility(Vol.4)Insolubleinwater,slightlysolubleinethanolUV/VISabsorptionThesampleinacetoneshowsabsorbancemaximaatabout425,457and(Vol.4)487nm.ThinLayerChromatographyActivateaTLCplate(e.g.LK6DSILICAGEL
7、60A(layerthickness:250µm,size:5x20cm))for1hat110˚.Preparea5%solutionofthesamplein95%ethanolandapply10µltotheplate.Allowtodryanddevelopusingamixtureofn-butanol,methylethylketoneand10%aqueousammonia(3:2:2byvolume)untilthesolventfronthasascendedabout10cm.Allowtodry.
8、BixinandnorbixinappearasyellowspotswithRfvaluesofabout0.50to0.45,respectively.Spraywith5%sodiumnitritesolutionandthenwith0.5mol/lsulfuricacidandthespotsimmedia
