冠状病毒Coronavirusl论文-2011 Genetically Diverse Coronaviruses in Captive Bird Populations in a Brazilian Zoological Park.pdf

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1、VECTOR-BORNEANDZOONOTICDISEASESVolume11,Number2,2011ªMaryAnnLiebert,Inc.DOI:10.1089/vbz.2010.0090GeneticallyDiverseCoronavirusesinCaptiveBirdPopulationsinaBrazilianZoologicalPark1212TerezaC.Cardoso,MariaCecı´liaB.Teixeira,DerianeE.Gomes,andAntoˆnioJose´JerezAbstractTh

2、isstudyaimedtoinvestigatetheoccurrenceofcoronaviruses(CoVs)incaptivebirdsplacedinsideazoo-logicalparkinBrazil.TheroleofcaptivebirdsintheepidemiologyofCoVsinthetropicsispoorlyunderstood.Atotalof25(n¼25)differentspeciesweretestedforviralRNAusingindividualfecalsamplescol

3、lectedfromhealthybirds.Reversetranscriptionpolymerasechainreactiontargetingthe30untranslatedregionwasusedtodetectCoVRNA,andpositivesamplesweresubmittedforsequenceanalysis.Thephylogeneticsearchrevealedninemutationsintheblackshoulderedpeafowl(Pavuscristatus)CoVsequence,

4、whichclusteredseparatelyfromsamplespreviouslydescribedinEngland.ThisisthefirstreportonthedetectionoftheCoVgenomeincaptivebirdsinBrazil.KeyWords:BrazilCaptivebirdsCoronavirusZooparks.Introductionstudyofwildlifediseasesincreases,itislikelythatnewviruseswillbediscoveredan

5、dnewdiseaseswillbeencoun-Theoutbreaksofhighlypathogenicavianinfluenzatered(Hughesetal.2009).Therefore,studiesoftheviraldis-virusesaroundtheglobeinthepastfewyearshavebeeneasesofwildandcaptivebirdsarenecessary.Thelatestdevastatingforthecountriesconcernedandhavecreatedtec

6、hniquesofmolecularepidemiologycanhelpscientistsfearinthegeneralpopulation.Similarly,in2002theworldunderstandhowvirusesmovefromspeciestospeciesandwitnessedtheemergenceofsevereacuterespiratorysyn-fromregiontoregion(Hughesetal.2009,Jackwoodetal.dromeinGuangdongProvince,C

7、hina,causedbyaprevi-2010).ouslyunknowncoronavirus(CoV)(Liu2003).ItislikelythatThepresentstudydemonstratesthemoleculardetectionadditional,yettobeidentifiedCoVsarecirculatingindo-andcharacterizationofvirusesfromcaptiveexoticbirdpop-mesticbirds,especiallyintropicalcountri

8、es.Thethreeex-ulationssampledinSaoPauloStateZoologicalParkinBrazil,istinggroupsofCoVswereinitiallydistinguishedbasedonwhicha