冠状病毒Coronavirusl论文-2001 The effects of coronavirus on human nasal ciliated respiratory epithelium.pdf

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1、EurRespirJ2001;18:965–970Copyright#ERSJournalsLtd2001DOI:10.1183/09031936.01.00093001EuropeanRespiratoryJournalPrintedinUK–allrightsreservedISSN0903-1936Theeffectsofcoronavirusonhumannasalciliatedrespiratoryepithelium#M.A.Chilvers*,M.McKean*,A.Rutman*,B.S.Myint,M.Silverman*,C

2、.O9Callaghan*Theeffectsofcoronavirusonhumannasalciliatedrespiratoryepithelium.M.A.*ChildrensAsthmaCentre,DeptofChilvers,M.McKean,A.Rutman,B.S.Myint,M.Silverman,C.O9Callaghan.#ERSChildHealth,UniversityofLeicester,#JournalsLtd2001.LeicesterRoyalInfirmary,andDeptABSTRACT:Humancor

3、onavirus(HCoV)accountsfor1530%ofcommoncolds,butofMicrobiologyandImmunology,UniversityofLeicester,Leicester,UK.onlyonecasereporthasdescribedtheeffectofacoronavirusinfection,thatwasasymptomatic,onhumanrespiratoryepithelium.Correspondence:C.O9Callaghan,Theauthorsexaminedtheeffec

4、tsofinfectionwithHCoVonciliarystructureandChildrensAsthmaCentre,DeptoffunctioninhealthyvolunteersinfectedbyintranasalinoculationwithHCoV229E.AChildHealth,UniversityofLeicester,furtherfourvolunteerswereshaminfectedwithultraviolet-inactivatedvirus.Immedi-RobertKilpatrickClinica

5、lSciencesatelybeforeinoculation(day0)and3dayslater(day3),ciliatedepitheliumwasobtainedBuilding,LeicesterRoyalInfirmary,bybrushingtheinferiornasalturbinate.Ciliarybeatfrequencywasdeterminedand6694,P.O.Box65,LeicesterLE27LX,beatpatternanalysedforevidenceofdyskinesia(0=normal,3=s

6、everelydyskinetic)usingUK.Fax:441162523282digitalhigh-speedvideophotography.Ciliaryultrastructurewasexaminedbytrans-missionelectronmicroscopy.Symptomdiarieswerekeptforthedurationofthestudy.Keywords:CiliaAllsubjectsinoculatedwithHCoV,includingthethreewhodidnotdevelopsymptomsci

7、liarybeatfrequencyofanupperrespiratorytractinfection,haddisruptionoftheirrespiratoryepitheliumcoronavirusonday3.Althoughtherewasnodifferenceinthemeanciliarybeatfrequencybetweenrespiratoryepitheliumday0(11.3Hz(95%confidenceinterval(CI):8.614.0)andday3(9.4Hz(95%CIultrastructure7

8、.211.6)),therewasasignificantincrease(pv0.05)intheciliarydyskinesiasc