资源描述：
《冠状病毒Coronavirusl论文-1994 Antigen detection in human respiratory Coronavirus infections by monoclonal time-resolved fluoroimmunoassay.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、~ClinicalandDiagnosticClinicalandDiagnosticVirologyVirologyELSEVIER2(1994)165-179AntigendetectioninhumanrespiratoryCoronavirusinfectionsbymonoclonaltime-resolvedfluoroimmunoassayJohnC.Hierholzer"'*,PekkaE.Halonena,b,PatriciaG.Bingham",RichardA.Coombs",YvonneO.Stone""R
2、espiratoryandEntericVirusesBranch,DivisionofViralandRickettsialDiseases,CenterforInfectiousDiseases,CentersforDiseaseControl,1600°CliftonRd.N.E.,Atlanta,GA30333,USA;bDepartmentofVirology,UniversityofTurku,Kiinamyllynkatu13,SF-20520,Turku52,FinlandReceived27May1993;rev
3、ised18November1993;accepted1December1993AbstractBackground:Thediagnosisofrespiratoryinfectionsbydetectingviralantigenshasreceivedconsiderableattentionusingimmunofluorescentassays(IFA)andenzymeimmunoassays(EIA).Time-resolvedfluoroimmunoassay(TR-FIA)hasbeendevelopedfors
4、everalviruses.Objectives:Topreparemonoclonalantibodiestocoronavirusstrains,toincorporatethemintoaTR-FIA,andtesttheassayonclinicalspecimens.Studydesign:MonoclonalantibodieswerepreparedtotheNnucleoproteinofthetwohumanrespiratorycoronaviruses,HCVstrains229EandOC43.Monocl
5、onalstobothviruseswerecompletelytype-specific;theydidnotcross-reactbetweenthemselvesorwithmultiplestrainsofotherrespiratoryviruses.TheseantibodieswereconfiguredintooptimizedEIAandTR-FIAtests.Theall-monoclonaltestswerethencomparedtopolyclonalEIAtestsintermsoftheirabili
6、tytodetectvirusinclinicalspecimens.Results:Theall-monoclonalTR-FIAwasuniformlythemostsensitive,detectingvirusinall13229E-positivespecimenscomparedto69%forthemonoclonalEIAand54%forthepolyclonalEIAtest.Similarresultswereobtainedfor10OC43-positivespecimens:100%inTR-FIA,9
7、0%inmonoclonalEIA,and80%inpolyclonalEIA.For229EinTR-FIA,meanpositive/negative(PfN)ratioswere143for229E-positivehumanembryoniclungfibroblast(HLF)cellculturefluidsand10forpositivenasopharyngealaspiratespecimens;forOC43inTR-FIA,meanP/Nvalueswere964forOC43-positiverhabdom
8、yosarcoma(RD)cellculturefluidsand174forpositiveNPAspecimens.ThesensitivitiesoftheTR-FIAweredeterminedwithpurifiedvirionstobe
