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1、DetectionofCoronavirusRNAandAntigeninMultipleSclerosisBrainRonaldS.Murray,MD,*BonnieBrown,BS,'DavidBrian,PhD,DVM,?andGaryF.Cabirac,PhD"$Epidemiologicalstudiesofpatientswithmultiplesclerosis(MS)andanimalmodeldatasupportthehypothesisthatvirusesinitiatetheim
2、munopathogeniceventsleadingtodemyelinationinMS.Therehavebeennoreports,however,ofconsistentdetectionofvirusesinMScentralnervoussystemtissue.WeprobedMSandcontrolbrainwithcDNAprobesspecificforhuman,murine,porcine,andbovinecoronaviruses.Wereporttheinsituhybri
3、dizationdetectionofcoronavirusRNAin12of22MSbrainsamplesusingclonedcoronaviruscDNAprobes.Inaddition,tissuewasscreenedforcoronavirusantigenbyimmunohistochemicalmethods;antigenwasdetectedintwopatientswithrapidlyprogressiveMS.Significantamountsofcoronavirusan
4、tigenandRNAwereobservedinactivedemyelinatingplaquesfromthesetwopatients.ThesefindingsshowthatcoronavirusescaninfectthehumancentralnervoussystemandraisethepossibilitythatthesevirusesmaycontributetothepathogenesisofMSinsomepatients.MurrayRS,BrownB,BrianD,Ca
5、biracGF.DetectionofcoronavirusRNAandantigeninmultiplesclerosisbrain.AnnNeurol1992;31:525-533Multiplesclerosis(MS)isachronicinflammatoryde-CoronaviruseshavebeenimplicatedinMSasare-myelinatingdiseaseofthehumancentralnervoussys-sultofthereportofvirusisolatio
6、nfromthebrainstem(CNS)withoutknowncause.ApopulartheoryoftwopatientswithMSandtheelectronmicroscopicproposesthatanexogenousstimulusinitiatesanim-observationofacoronavirusinanMSbrainperivascu-muneresponseagainstendogenousCNSproteins,pos-larimmunocyte17,83.Su
7、pportfortheideathatcoro-siblymyelinstructuralproteins.However,damagetonaviruseshavearoleinMScomesfromdatashowingtheCNSastheresultofanimmuneresponsetoathatthesevirusescausedemyelination[9}andarecapa-chroniclow-gradeCNSinfectionhasneverbeenruledbleofstimula
8、tingT-cell-mediatedautoimmunereac-out.Therefore,demyelinatinglesionsinMSmayrep-tionsinrodents{lo,11).Althoughthesestudiesareresentthefinaldirectorindirectimmunopathologicalintriguing,moreevidenceisneededtoconfirmtha
