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1、VeterinaryMicrobiology,26(1991)25-4025ElsevierSciencePublishersB.V.,AmsterdamTheuseofenzyme-linkedimmunosorbentassaysystemsforserologyandantigendetectioninparvovirus,coronavirusandrotavirusinfectionsindogsinTheNetherlandsG.F.Rimmelzwaana,J.Groena,H.Egberinkb,G.H.A.Borstc,F.G.C.M.Uytd
2、eHaagaandA.D.M.E.OsterhausaaLaboratoryoflmmunobiology,NationalInstituteofPublicHealthandEnvironmentalProtection.P.O.BoxI,3720BABilthoven,NetherlandsbDepartmentofVirology,VeterinaryFaculty,StateUniversityofUtrecht,YalelaanI,3508TDUtrecht,Netherlands"RegionalAnimalHealthServiceUentre,Z
3、wartewaterallee8,8031DXZwolle,Netherlands(Accepted16May1990)ABSTRACTRimmelzwaan,G.F.,Groen,J.,Egberink,H.,Borst,G.H.A.,UytdeHaag,F.G.C.M.andOsterhaus,A.D.M.E.,1991.Theuseofenzyme-linkedimmunosorbentassaysystemsforserologyandantigendetectioninparvoviruscoronavirusandrotavirusinfection
4、sindogsinTheNetherlands.Vet.Microbiol.,26:25-40.Complextrappingblocking(CTB)enzyme-linkedimmunosorbentassays(ELISAs)andindirectELISAsforthedetectionofantibodiestocanineparvovirus(CPV),caninecoronavirus(CCV)androtavirusinseraofdogswereestablished.DoubleantibodysandwichELISAsforthedete
5、ctionofCPV-,CCV-androtavirusantigensinfecalsampleswerealsodeveloped.Boththeserologicalandantigen-detectionELISAswereusedtoscreensamplesfromdogsinTheNetherlands,withorwithoutahistoryofacutediarrhea.ItwasshownthattheresultsoftherespectiveserologicalELISAscorre-latedwellandthatCPVwasthe
6、majorcauseofvirus-inducedacutediarrheaindogsinTheNetherlands.INTRODUCTIONThreedifferentviruses,canineparvovirus(CPV),caninecoronavirus(CCV)androtavirus,havebeenshowntobeassociatedwithacutegastroin-testinaldiseaseanddiarrheainthedog(Keenanetal.,1976;Appeletal.,1978;Appeletal.,1979a,b;
7、EugsterandSidwa,1979;EnglandandPoston,1980;Evermannetal.,1980;McNultyetal.,1980;Osterhausetal.,1980b;Vandebergheetal.,1980;Williams,1980;Fultonetal.,1981;Johnsonetal.,1983).Therelativeroleofeachofthesevirusesincausinggastrointestinaldiseaseinthisspecieshassofarattractedlimitedattenti
8、on(Osterhaus
