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1、Comp.Immun.Microbiol.infect.Dis.Vol.12,No.4,pp.139146,19890147-9571/89$3.00+0.00PrintedinGreatBritain.AllrightsreservedCopyright©1989PergamonPressplcANENZYME-LINKEDIMMUNOSORBENTASSAYUSINGCANINECORONAVIRUS-INFECTEDCRFKCELLSASANTIGENFORDETECTIONOFANTI-CORONAVIRUSANTIB
2、ODYINCATMASAMIMOCHIZUKI*andHISASHIFURUKAWALaboratoryofVeterinaryMicrobiology,DepartmentofVeterinaryMedicine,FacultyofAgriculture,KagoshimaUniversity,Korimoto1-21-24,Kagoshima890,Japan(Received13July1989)Abstract--Fromthereasonsthatcaninecoronavirus(CCV)growsmoreeffi
3、cientlythanfelinecoronavirusinacellcultureandtheyaremutuallyrelatedintheirantigenicities,anenzyme-linkedimmunosorbentassay(ELISA)usingCCV-infectedfelinekidney(CRFK)cellsassubstrateantigenswasdevelopedfordetectionofanti-coronavirusantibodiesincats.Itwasindispensablef
4、orgeneratingcoronavirus-specificELISAantibodyactivitiesthatthesamplewasappliedtothemock-infected,normalCRFKcellsinparallelwiththeCCV-infectedcellsandthentheopticaldensityvaluesgivenbythemock-infectedcellantigenweresubtractedfromthosegivenbythevirus-infectedcellantig
5、en.OnthebasisofELISAantibodytitersobtainedinserafromthecatsexperimentallyinfectedwithCCVandfromthespontaneousfelineinfectiousperitonitis(FIP)cases,theELISAdescribedinthepresentstudywasfoundtobeapplicableasasimpleandeasyserologictestwhichwasabletodetectanti-coronavir
6、usantibodiesasefficientlyastheindirectimmunofluorescenceassaywithhomologousFIPvirus.Keywords:Coronavirus,felineinfectiousperitonitisvirus,caninecoronavirus,enzyme-linkedimmunosorbentassay,ELISA.R6sum~-Etantdonn6quecoronaviruscanin(CVC)prolif&eenculturecellulaireavec
7、unemeilleureefficacit6quecoronavirusf61inetqu'ilsontuneantig6n6citeorois6e,untestELISAutilisantdescellulesr6nalesf/flines(CRFK)infect6esparleCVCcommeantig6nesubstrata6t6d6v61op6epourlad&6ctiondesanticorpsanti-coronavirauxchezlechat.Pourobteniruneactivit6anticorpsELI
8、SAcoronavirus-sp&ifique,ila6t6consid6r6commeindispensablequel'&hantillonsoitappliqu6auxcellules(CRFK)normales,noninf&t&senparall61eavecles
