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《冠状病毒Coronavirusl论文-1990 Sequence comparison of the N genes of five strains of the coronavirus mouse hepatitis virus suggests a three domain.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、VIROLOGY179,463-468(1990)SequenceComparisonoftheNGenesofFiveStrainsoftheCoronavirusMouseHepatitisVirusSuggestsaThreeDomainStructurefortheNucleocapsidProteinMONICAM.PARKERANDPAULS.MASTERS’WadsworthCenterforLaboratoriesandResearch,NewYorkStateDepartmentofHealth,Albany,NewY
2、ork1220l-0509ReceivedMay2,1990;acceptedJuly11,1990Toobtaininformationaboutthestructureandevolutionofthenucleocapsid(N)proteinofthecoronavirusmousehepatitisvirus(MHV),wedeterminedtheentirenucleotidesequencesoftheNgenesofMHV-A59,MHV-3,MHV-S,andMHV-1fromcDNAclones.Atthenucl
3、eotidelevel,theNgenesequencesoftheseviralstrains,andthatofMHV-JHM,weremorethan92%conservedoverall.Evenhighernucleotidesequenceidentitywasfoundinthe3’untranslatedregions(3’UTRs)ofthefivestrains,whichmayreflecttheroleofthe3’UTRinnegative-strandRNAsynthesis.AllfiveNgeneswer
4、efoundtoencodemarkedlybasicproteinsof454or455residueshavingatleast94%sequenceidentityinpairwisecomparisons.However,aminoacidsequencedivergenceswerefoundtobeclusteredintwoshortsegmentsofN,putativespacerregionsthat,together,constitutedonly11%ofthemolecule.Thus,thedatasugge
5、stthattheMHVNproteiniscomposedofthreehighlyconservedstructuraldomainsconnectedtoeachotherbyregionsthathavemuchlessconstraintontheiraminoacidsequences.Thefirsttwoconserveddomainscontainmostoftheexcessofbasicaminoacidresidues;bycontrast,thecarboxy-terminaldomainisacidic.Fi
6、nally,wenotedthatfourofthefiveNgenescontainaninternalopenreadingframethatpotentiallyencodesaproteinof207aminoacidshavingalargeproportionofbasicandhydrophobicresidues.o1990AcademicPUSS,IIIC.Coronavirusesareafamilyofenveloped,single-sis(SDS-PAGE)(datanotshown;Refs.(8-IO)),
7、sug-stranded,positive-senseRNAvirusesthatareimpor-gestingdifferencesinproteinsizeoraminoacidcom-tantrespiratory,neurologic,andentericpathogensforposition.humansanddomesticanimals(1).HavingthelargestMultiplecDNAclones,preparedfrompoly(A)-con-genomiccodingcapacitiesamongRN
8、Aviruses(attainingRNAfrominfectedmouse17clone1cells,wereleast27kb)aswellasauniquestrategyofRNAreplica-u
