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1、JournalofGeneralVirology(1992),73,3285-3288.PrintedinGreatBritain3285DifferentialinvitroinhibitionoffelineentericcoronavirusandfelineinfectiousperitonitisvirusbyactinomycinDEvelynL.Lewis,1D.A.Harbour,1.J.E.Beringer2andJ.Grinsted31DepartmentofVeterinaryMedicine,LangfordHouse,BristolBS187DU,2Departm
2、entofBotanyandZoology,WoodlandRoad,BristolBS81UGand3DepartmentofPathologyandMicrobiology,MedicalSchool,UniversityofBristol,UniversityWalk,BristolBS81TD,U.K.Thegrowthoffelineentericcoronavirusstrain79-1683relatedfelineinfectiousperitonitisvirusstrain79-1146inwholefelineembryocellswasinhibitedbythew
3、asunaffectedbythepresenceofactinomycinD,presenceof1~tg/mlofactinomycinDinthecultureindicatingafundamentaldifferencebetweenthetwofluid.Novirus-specificmRNAscouldbedetectedinfelinecoronavirusstrainsintheirrequirementsforsuchculturesandyieldsofinfectiousviruswerehost-encodedfunction(s).depressedby>99
4、%.Bycontrast,theantigenicallyFelineentericcoronavirus(FECV)andfelineinfectiousgrownin25cm2flaskswereinoculatedwith10p.f.u./cellperitonitisvirus(FIPV)aretwocoronaviruseswhich,ofeitherFECV79-1683orFIPV79-1146.After1halthoughantigenicallysimilar(Sanchezetal.,1990),incubationat37°C,growthmediumwithorw
5、ithoutshowmarkedlydifferenttissuetropismsandvirulence.1~tg/mlactinomycinDwasaddedandthecultureswereFECVcausesamildorinapparententeritisinkittensreincubatedat37°C.Mock-infectedWFEcellswerewhereasFIPVinducesafatal,immune-mediatedprocessedsimultaneously.After6h,totalcellularRNAperitonitis(Pederseneta
6、l.,1981;Lutzetal.,1986;waspreparedbyextractionwithguanidiniumisothio-Evermannetal.,1991).Thecriticaleventinthecyanate,andpelletedthroughacaesiumchloridesteppathogenesisofFIPV-mediateddiseaseappearstobethegradient(Maniatisetal.,1982).ViralintracellularRNAsabilityofthevirustoinfectmacrophagesproduct
7、ivelywerevisualizedbyNorthernblothybridizationsusinga(Stoddart&Scott,1989).FECVisunabletodosocDNAproberepresentingthe3'endoftheFIPVefficientlyandremainslocalizedintheepithelialcellsofgenome.RNApreparationswereele
