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1、ArchivesofVirologyArchivesofVirology85,71--83(1985)©bySpringer-Verlag1985StudiesontheRelationshipBetweenCoronavirusesfromtheIntestinalandRespiratoryTractsoiCalvesByD.J.REYNOLDS,T.G.DEBNEY,G.A.HALL,L.H.THO~AS,andK.t~.PAnso~sA.F.1%.C.Iastitutefor1%esear
2、chonAnimalDiseases,Compton,Newbury,Berkshire,UnitedKingdomWith3FiguresAcceptedNovember1,1984SummaryAnimmunofluorescencetestonsmearsofnasalepithelialcellswasusedtodetectcoronavirusinfectionintherespiratorytractofcalves.Thirteengnotobioticcalveswereinfe
3、ctedwithcoronavirusisolatesderivedfromfaecesorrespiratorymaterial:viruswasdetectedinfaecesandnasalswabsfromallanimals.In115calvesfromafieldsurvey,therewasasignificantassociationbetweencoronavirusexcretionfrombothrespiratoryandentericroutesincalveswith
4、diarrhoea.Inafurther12calves,atnecropsy,thepredilectionsitesforcoronavirusgrowthwerethedistalsmallintestine,largeintestineandtheepitheliaofthenasalcavityandtrachea.Antigenwasnotfoundinlungtissuebyimmunofluorescenceorimmunoperoxidasestaining.Infectionw
5、ithentericcoronavirusinducedimmunitytoreinfectionandtoheterologouschallengewithtwocoronavirusisolatesderivedfromtherespiratorytract.Ninecoronaviruseswerecultivated,clonedandantiseratothreewerepreparedinpigs.Therewascompletevirusneutralisationintestswi
6、thhomologousseraandsignificantcrossreactionswiththeeightotherisolateswhichwereofintestinalandrespiratoryorigin.Thus,thesebovinecoronavirusisolatesbelongedtothesameserotypedespitethesourceofvirus.Introdueti.nCoronavirusareimportantcausesofupperrespirat
7、orytractillness,encephalitis,hepatitisandentericdiseaseinhumansoranimals(27).The72D.J.REY~'OLDSetal.:intestineistheprimarysiteforreplicationoftransmissiblegastroeuteritisvirus(TGEV)ofpigs,canineandbovinecoronavirus(BCV).Virusreplicationcausespathologi
8、calchangesintheintestinalvillianddiarrhoeacanresult(10).Threecoronavirusesareconsideredtoberespiratorypathogens(14,21);theyareavianinfectiousbronchitisvirus(AIBV),ratcoronavirus(RCV)andhumancoronavirus(HCV).Cultureadapted(20)andfieldstrains(1)
