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1、ArchivesofVirologyArchivesofVirology54,107--117(1977)©bySpringer-Verlag1977FurtherStudiesonHumanEntericCoronavirusesByE.O.CAuL1andS.I.EGGLESTONE21PublicHealthLaboratory,Bristol,England2SchoolofEnvironmentalSciences,PlymouthPolytechnic,Plymouth,EnglandW
2、ith6FiguresAcceptedFebruary4,1977SummaryComparisonsweremadebetweenhumanentericcoronavirusesandtheentericcoronavirusesofpigsandcalvesbynegativestaining.Examinationofhumanintestinalorganculturefluidsatvarioustimeintervalsafterinoculationwiththehumanenter
3、iccoronavirusshowedincreasingnumbersofparticlesinthefluids.Thinsectionsofthecolumnarepithelialcellsoftheseexplantsshowedanum-beroffeaturesconsistentwiththereplicationofknownhumanandanimalcorona-viruses.Virusparticlesfoundinthinsectionshadameandiametero
4、f68nm.Inaddition,astructurewasfoundinthinsectionswhichhasnotbeendescribedpreviously.Thisstructuremayrepresenttheviralnueleocapsid.IntroductionHumancoronaviruseshavebeenisolatedinhumanfoetaltrachealandnasalepitheliumorgancultureandinrnonolayercellcultur
5、es(1,4,10,12,16,20).Virionsobtainedfromtheseculturesshowedthetypicaleoronavirusmorphologybynegativestaining(3).Humancoronaviruseshavebeenstudiedextensivelybynegativestainingofcellandorganculturefluidsbutuntilrecentlyhavenotbeenseenordescribedinclinical
6、material(6).UltrastructurMchangesfollowingrepli-cationofcoronavirusesincellcultureshavebeenreportedbyanumberofworkers(15).However,therearenosimilarreportswhichdescribeultrastructurMchangesfollowingreplicationinhumanorgancultures.Thepresentelectronmicro
7、scopicalinvestigationwasundertakentostudythemorphologybynegativestainingofthenewlydescribedhumanentericcoronaviruses(5)andtocomparethemwithtwoanimalentericcoronaviruses,namely,transmissiblegastroenteritisvirus(TGEV)ofpigsandneonatalcalfdiarrhoeavirus.I
8、nadditionobservationsweremadeontheultrastructuralchangesassociatedwiththereplicationofthishumanentericcoronavirusinhumanfoetalintestinalorgancultures.Infectionoftheorgancul-tureswiththisnon-laboratoryadaptedvirusrepresentsamodelsystemas
