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1、VeterinaryMicrobiology,6(1981)143--155143ElsevierScientificPublishingCompany,Amsterdam--PrintedinTheNetherlandsINHIBITORSOFBOVINEPARVOVIRUS,CORONAVIRUSANDROTAVIRUSINPRECOLOSTRALANDFETALBOVINESERAS.TOKUHISA,Y.INABA,K.SATO,Y.MIURA,H.AKASHI,K.SATODAandM.MATUMOTO*NationalInstituteofAnimalHealth,Tsukuba,
2、Ibaraki(Japan)*KitasatoInstitute,Minato-ku,Tokyo(Japan)(Accepted12January1981)ABSTRACTTokuhisa,S.,Inaba,Y.,Sato,K.,Miura,Y.,Akashi,H.,Satoda,K.andMatumoto,M.,1981.Inhibitorsofbovineparvovirus,coronavirusandrotavirusinprecolostralandfetalbovinesera.Vet.Microbiol.,6:143--155.Eleven,11and2of11precolost
3、ralserafromnormalcalvesand13,14and9of14serafromnormalfetuses,7to10monthsofgestation,neutralizedbovineparvovirus,coronavirusandrotavirus,respectively.Whenassayedbysingleradialimmunodiffusion,alltheseracontainedIgGat360to1400mg/dl,andsomeofthemhadmuchsmalleramountsofIgMorIgA.Mostoftheneutralizingactiv
4、itiesagainstbovinecoronavirusandrotaviruswerereadilyinactivatedbytreatmentwithacetoneor2-mercaptoethanol.SomeserafrectionatedbySephadexG-200gelfiltrationorstarchblockelectrophoresishadneutralizingactivitiesagainstbovineparvovirusorcoronavirusinfractionscontainingnodetectableamountsofimmunoglobulins.
5、Theseobservationsseemtoindicatethepresenceofsubstance(s),otherthanimmunoglobulins,capableofinhibitingreplicationofbovineparvovirus,coronavirusorrotavirus.Thechemicalnatureandthemodeofactionoftheinhibitorsawaitelucida-tion.INTRODUCTIONTransplacentaltransferofmaternalimmunoglobulinsdoesnotoccurincattl
6、e,andthenewbornanimalacquiresmaternalantibodiesbyingestingcolostrum(Brambell,1970;Solomon,1971).Ontheotherhand,bovinefetusesdeveloptheabilitytoproduceantibodiesuponantigenicstimulationrelativelyearlyingestation(Solomon,1971;Osburn,1973;Schultz,1973;Schultzetal.,1973).Manyworkershavereportedthepresen
7、ceofantibodiesagainstvariousvirusesinserafromcolostrum-deprivedcalvesandfetuses(Kniazeffetal.,1967;Dunneetal.,1973;Homeretal.,1973;Hubbertetal.,1973;Schultzetal.,1973;Miuraetal.,1974;RossiandKiesel,19
