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1、ArchivesofVirologyArchivesofVirology67,367---370(1981)©bySpringer-Verlag1981PropagationoftheKakegawaStrainofBovineCoronavirusinSueklin9Mice,RatsandHamstersBriefReportByH.AKASHI1,Y.INABA1,Y.MIURA1,K.SATO1,S.TOKUttlSAt,M.ASAGI2,andY.I-IAYAStlI31NationalInstituteofAnimalHealth,Tsukuba,Japan2NIl-I
2、ONVACCNECo.Lgd.,Nishinasuno,Tochigi,JapanaZEN-NOI{CentralInstituteforFeedandLivestockResearch,Tsukuba,JapanW'ith1FigureAcceptedNovember5,1980SummaryTheKakegawastrainofbovinecoronaviruswaseasilypropagatedinsucklingmice.Infectedanimalsdiedwithnervoussymptoms,andserialpassagewasreadilyaccomplishe
3、dbyintraeerebralinoculationwithbrainemulsions.The3rdpassageviralmaterialfrominfectedmiceevokedthesamediseaseinsucklingmice,ratsandhamstersinoculatedbytheintracerebralorbythesubcutaneousroute.Virusesrecoveredfrommice,ratsandhamsterscouldbeclearlydifferentiatedfrommousehepatitisvirusstrain2bythe
4、neutralizationtest.Abovinecoronavirus(BCV)hasbeenrecognizedasoneofthecausativeagentsofdiarrheaincalves(8).l~ecentlytheKakagawastrainisolatedfromthefecesofacowwithcpizooticdiarrheahasbeenidentifiedasBCV(1,9).However,thestudyofbovinediarrhealdiseaseproducedbycoronavirushasbeengreatlyhamperedbydi
5、fficultyinisolatingvirusincellculturesandthelackofexperimentalhostsotherthancattle.Recentlymanymembersoftheeorona~drusfamily"havebeenfoundtogrowinsucklingmice(5--7).ThepresentpaperdescribesbrieflyourrecentobservationthattheKakegawastrainofBCVreadilypropagatesinsucklingmice,ratsandhamsters.TheK
6、akegawastrainofBCVusedformouseinoculationwasat,the10thpassagelevelinprimarybovinekidneycellcultures.Conventionallyrearedone-day-oldmice(strainddY),ratsandSyrianhamsterswereeachinoculatedwith0.01-mloftheviralmaterialsbytheintracerebral(ic)routeandwith0.02-mlby25b*0304-8608/81/006710367/$0t.0036
7、8H.A~:As~Ietal.:thesubcutaneous(s.c.)route.Virusrecoveryandinfectivityassaywerecarriedout.byinoculationintocellculturesofBEK-1ecll,derivedfrombovineembryonickidney(3).Thea:nti-Kakegawastrainimmuneserumhavingahomologousneu-tralizingantib