应用响应面法优化桑叶多糖_黄酮和生物碱联合提取工艺条.pdf

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1、ScienceofSericulture蚕业科学2013，39(3):0568－0575ISSN0257－4799;CN32－1115/SE-mail:CYKE@chinajournal．net．cn应用响应面法优化桑叶多糖、黄酮和生物碱联合提取工艺条件刘凡黄勇廖森泰肖更生邹宇晓施英穆利霞刘军沈维治(广东省农业科学院蚕业与农产品加工研究所，广东省农产品加工重点实验室，广州510610)摘要为了高效提取桑叶活性物质，采用热水浸提桑叶中的多糖和黄酮后，再用乙醇-盐酸溶液进一步提取桑叶生物碱，并通过响应面法优化3类桑叶生物活性物质的联合提取工艺条件。优化后的桑叶多糖和

2、黄酮提取工艺条件为提取时间93min、提取温度81℃、料液质量浓度0.05g/mL、提取次数4次，在此条件下实测桑叶多糖和黄酮的提取得率分别为15.574%、2.693%;优化后的桑叶生物碱提取工艺条件为提取时间142min、料液质量浓度0.04g/mL、乙醇体积分数26.0%、提取次数3次，在此条件下实测桑叶生物碱的提取得率为0.398%。建立的回归模型拟合性好，可用于预测桑叶活性物质提取得率。关键词桑叶;多糖;黄酮;生物碱;工艺条件;响应面法中图分类号S888;S886．9;R284．2文献标识码B文章编号0257－4799(2013)03－0568－08P

3、rocessOptimizationforCombinedExtractionofPolysaccharide，FlavonesandAlkaloidfromMulberryLeavesbyResponseSurfaceMethodology*LIUFanHUANGYongLIAOSen-TaiXIAOGeng-ShengZOUYu-XiaoSHIYingMULi-XiaLIUJunSHENWei-Zhi(Sericulture＆Agri-foodResearchInstitute，GuangdongAcademyofAgriculturalSciences，K

4、eyLaboratoryofAgro-foodScienceandTechnologyofGuangdong，Guangzhou510610，China)AbstractToobtainactivesubstancesfrommulberryleavesefficiently，wefirstextractedpolysaccharideandflavonesfrommulberryleafbyhotwatermethod，andthenextractedalkaloidfrommulberryleafusingethanol-hydrochloricacidso

5、lution．Thecombinedprocessforextractingthesethreeactivesubstancesfrommulberryleafwasoptimizedbyre-sponsesurfacemethodology．Theoptimizedconditionforextractingpolysaccharideandflavonesfrommulberryleafwasextractiontime93min，extractiontemperature81℃，massconcentrationofrawmaterial0.05g/mL，

6、and4timesofex-traction．Underthiscondition，theactualextractionyieldofpolysaccharideandflavoneswas5.574%and2.693%，re-spectively．Theoptimizedconditionforextractingalkaloidfrommulberryleafwasextractiontime142min，massconcen-trationofrawmaterial0.04g/mL，volumefractionofethanol26.0%，and3tim

7、esofextraction．Underthiscondition，theactualextractionyieldofmulberryleafalkaloidwas0.398%．Theresultindicatedthattheestablishedmodelhadgoodfit-tingperformance，andcouldbeusedtopredictthe收稿日期:2013－03－17接受日期:2013－03－25extractionyieldofactivesubstancesfrommulberryleaves．资助项目:现代农业产业技术体系专项(

8、CARS-22)，国家自