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1、致倦库蚊主要组织结构的石蜡切片观察闫亮珍54(3)：373-380昆虫学报ActaEntomologicaSinica,March2011,ISSN0454-6296致倦库蚊主耍组织结构的石蜡切片观察2211闫亮珍，乔传令，张建珍，马恩波，崔(1.Hl西大学应用生物学研究所，太原030006；2.中国科学院动物研究所，农业虫害鼠害综合治理研究国家重点实验室，北京100101)峰2,*摘耍：【冃的】蚊虫是传播人类多种疾病的重要媒介害虫，对其组织形态学的认识是开展众多领域研究的基础。本文通过研究致倦库蚊C
2、ulexpipiensquinquefasciatus成虫组织结构及形态，为媒介蚊虫的抗药性研究及有效防治提供基础o【方法】材料釆用改进的石蜡切片法和HE染色，结合活体内脏器官解剖及光学显微镜观察，从形态学和组织学水o【结果】获得结构完整、染色清晰、定位准确的消化排泄系统、生殖系统、神经平对致倦库蚊组织结构做详尽展示o【结论】系统、呼吸系统等HE染色石蜡切片探讨了改进制片和染色过程中一些步骤及注意事项。研究结果为利用原位杂交、免疫组化等方法研究蚊虫体内抗药性基因的准确定位及基因功能分析提供了可靠的基础
3、。关键词：致倦库蚊；石蜡切片；组织结构；解剖学；HE染色6296(2011)03-0373-08中图分类号：Q964文献标识码：A文章编号:0454-HistologicalobservationofmajorstructuresofthemosquitoCulexpipiensquinquefasciatus(Diptera：Culicidae)usingparaffintissuesection2*YANLiang-Zhenl,,QIAOChuan-Ling2,ZHANGJian-Zhenl,MA
4、En-Bol,CUIFeng2,(1.InstituteofAppliedBiology,ShanxiUniversity,TaiyuanO30006,China；2.StateKeyLaboratoryofIntegratedManagementofPestlnsects&Rodents,InstituteofZoology,ChineseAcademyofSciences,BeijinglOOlOl,China)Abstract：[Aim]Mosquitoesareimportantvectors
5、ofmanyhumandiseases.Theknowledgeoftheirhistologicalstructuresisthebasisofvariousresearches.ThehistologicalandmorphologicalstructuresofCulexpipiensquinquefasciatusexhibitedinthisstudyprovidethebasisfortheresearchofinsecticideresistanceandcontrolofmosquit
6、oes.【Methods]Improvedconsecutiveparaffintissuesections,Hematoxylin・Eosin(HE)stainingandorgandissectionwereperformedtostudythehistologicalstructuresofC・pipiensquinquefasciatusonthelevelsofmorphologyandhistology.【Results】Thedigestive,excretory,reproductiv
7、e,nervousandrespiratorysystemsofC.pipiensquinquefasciatuswereobtainedanddemonstratedclearly・【Conclusion】DetailedandimprovedprotocolsofparraffintissuesectionpreparationandHematoxylin-Eosinstainingwerediscussed.Thisstudyprovidestheinformationofhistologica
8、lstructuresofC.pipiensquinquefasciatus,whichwillshedlightontheexplorationofgenefunctionsofmosquitousinginsituhybridization,immunohistochemistry,etc.Keywords：Culexpipiensquinquefasciatus；paraffintissuesection；histologicalstructure
