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《复方中药合剂对AA肉仔鸡脾脏和法氏囊结构及发育的影响.pdf》由会员上传分享,免费在线阅读,更多相关内容在工程资料-天天文库。
1、第45卷第4期东北农业大学学报45(4):94-982014年4月JournalofNortheastAgriculturalUniversityApril2014网络出版时间2014—4—2113:22:46[URLIhttp://www.cnki.net/kcms/detail/23.1391.S.20140421.1323.014.html复方中药合剂对AA肉仔鸡脾脏和法氏囊结构及发育的影响靳双星,张桂枝(河南牧业经济学院畜牧工程系,郑州450046)摘要:选用7日龄健康AA肉仔鸡240只,随机分4组,每组设5个重复,对
2、照组自由饮水。试验组按0.5%、1%、1.5%的浓度添加复方中药合剂于饮水中,试验期为4周。分别于14日龄、21日龄、28日龄和35日龄,每组随机抽取10只鸡,颈静脉放血致死,取法氏囊和脾脏,测定脾脏和法氏囊指数。在免疫器官指数对比的基础上,选取效果最佳组和对照组的脾脏和法氏囊,固定,制作石蜡切片,H·E染色,显微观察、拍照。与对照组相比,试验组显著或极显著提高肉仔鸡21、2872.35日龄的脾脏指数和法氏囊指数(P3、显著高于对照组(P<0.05或P<0.01);并且脾脏结构清晰,白髓内的脾小体数量较多;脾动脉周围淋巴鞘明显增厚,鞘内淋巴细胞密集;法氏囊内淋巴小结数量较多,排列紧密,法氏囊皱襞发达,皱襞内淋巴细胞增多。说明复方中药合剂能显著促进AA肉仔鸡脾脏和法氏囊的发育,增强机体的免疫功能。关键词:复方中药合剂;免疫器官指数;脾脏;法氏囊中图分类号:$831.7文献标志码:A文章编号:1005—9369(2014)04—0094—05靳双星,张桂枝.复方中药合剂对AA肉仔鸡脾脏和法氏囊结构及发育的影响【J】_东北农业大学学报,2014,44、5(4):94-98.JinShuangxing,ZhangGuizhi.Efe~ofcompoundtraditionalchinesemedicinemixturesontissuestructureanddevelop-mentofspleenandfabriciusinbroilers[J].JournalofNo~heastAgriculturalUniversity,2014,45(4):94—98.(inChinesewithEnglishabstract)Efectofcompoundtraditionalch5、inesemedicinemixturesontissuestructureanddevelopmentofspleenandfabriciusinbroilers/JtNShuangxing,ZHANGGuizhi(AnimalDepartment,HenanUniversityofAnimalHusbandryandEconom~Zhengzhou450046,China)Abstract:240healthyAAbroilersattheageof7dayoldwereselected,anddividedintoexp6、erimentalandcontrolgroupsrandomly.Therewere5repeatinggroupsineachgroup.Thecontrolgroupwaswaterednormally,andtheexperimentalgroupswerewateredwithO.5%,1.0%and1.5%thecompoundtraditionalChinesemedicinemixtures,respectively.Thetrialperiodwas4weeks.On14,21,28and35daysolda7、ftergavaged,10chickensweresampledrandomlyfr0meachgroupandslaughteredbybloodlettinginjugularvein.ThenFabnciusandspleenswerecollectedfordeterminationofimmuneorgansindex.SpleensandFabnciuswererandomlyselectedfr0mthebestexperimentalgroupsandcontrolgroupandfixed.Pa阳怖nsec8、tionswerestainedwithHEandobservedundermicroscope.Theresultsshowedthaltheimmuneorganindexesweresignificantlyhigherintheexperimentalgroupsth
3、显著高于对照组(P<0.05或P<0.01);并且脾脏结构清晰,白髓内的脾小体数量较多;脾动脉周围淋巴鞘明显增厚,鞘内淋巴细胞密集;法氏囊内淋巴小结数量较多,排列紧密,法氏囊皱襞发达,皱襞内淋巴细胞增多。说明复方中药合剂能显著促进AA肉仔鸡脾脏和法氏囊的发育,增强机体的免疫功能。关键词:复方中药合剂;免疫器官指数;脾脏;法氏囊中图分类号:$831.7文献标志码:A文章编号:1005—9369(2014)04—0094—05靳双星,张桂枝.复方中药合剂对AA肉仔鸡脾脏和法氏囊结构及发育的影响【J】_东北农业大学学报,2014,4
4、5(4):94-98.JinShuangxing,ZhangGuizhi.Efe~ofcompoundtraditionalchinesemedicinemixturesontissuestructureanddevelop-mentofspleenandfabriciusinbroilers[J].JournalofNo~heastAgriculturalUniversity,2014,45(4):94—98.(inChinesewithEnglishabstract)Efectofcompoundtraditionalch
5、inesemedicinemixturesontissuestructureanddevelopmentofspleenandfabriciusinbroilers/JtNShuangxing,ZHANGGuizhi(AnimalDepartment,HenanUniversityofAnimalHusbandryandEconom~Zhengzhou450046,China)Abstract:240healthyAAbroilersattheageof7dayoldwereselected,anddividedintoexp
6、erimentalandcontrolgroupsrandomly.Therewere5repeatinggroupsineachgroup.Thecontrolgroupwaswaterednormally,andtheexperimentalgroupswerewateredwithO.5%,1.0%and1.5%thecompoundtraditionalChinesemedicinemixtures,respectively.Thetrialperiodwas4weeks.On14,21,28and35daysolda
7、ftergavaged,10chickensweresampledrandomlyfr0meachgroupandslaughteredbybloodlettinginjugularvein.ThenFabnciusandspleenswerecollectedfordeterminationofimmuneorgansindex.SpleensandFabnciuswererandomlyselectedfr0mthebestexperimentalgroupsandcontrolgroupandfixed.Pa阳怖nsec
8、tionswerestainedwithHEandobservedundermicroscope.Theresultsshowedthaltheimmuneorganindexesweresignificantlyhigherintheexperimentalgroupsth
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