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1、VIROLOGY185,847-852(1991)TheHemagglutinin/EsteraseGlycoproteinofBovineCoronaviruses:SequenceandFunctionalComparisonsbetweenVirulentandAvirulentStrainsXUMINGZHANG,KONSTANTING.KOUSOULU,ANDJOHANNESSTORZ’DepartmentofVeterinaryMicrobiologyandParasitology,SchoolofVeterinaryMedicine,LouisianaSta
2、teUniversity,BatonRouge,Louisiana70803ReceivedJuly9,1991;acceptedAugust21,1991Theentirenucleotidesequencesofthehemagglutinin/esterase(HE)genesspecifiedbythehighlyvirulentstrainLY138andtheavirulentstrainL9ofbovinecoronavirus(BCV)weredetermined.Thesesequenceswerecomparedwithrecentlypublishe
3、dsequencesoftheHEgenesoftheQuebecandMebusstrains.Alargeopenreadingframeof1272ntencodingaproteinof424aminoacidresidueswaspredicted.TheputativeesteraseactivesitewasconservedinthevirulentandavirulentBCVstrains,indicatingthatthisdomainisprobablynotadeterminantforBCVvirulence.Fouraminoacidsubs
4、titutionsoccurredbetweentheHEproteinsofBCV-L9andBCV-LY138(LeutoProat5,LeutoValat103,SertoProat387,andThrtoAsnat379).MonoclonalantibodiesspecificfortheHEglycoproteininhibitedthehemagglutinationandacetylesteraseactivitiesofBCV-L9,butshowednoinhibitoryeffectontheacetylesteraseactiv-ityofBCV-
5、LY138.Theseresultssuggestthatatleastoneepitopeislocatedproximaltooneofthethreestrain-specificaminoacids.FourS-specificmonoclonalantibodiesinhibitedhemagglutinationbutnotacetylesteraseactivityofBCV-L9,implyingthattheSglycoproteincanpromotehemagglutinationofchickenerythrocytesinadditiontoth
6、eHE!&COprOtein.Q1991AcademicPress,Inc.Bovinecoronavirus(BCV)isamemberoftheCoro-kDainitsreducedformandexistsasadisulfide-linkednaviridae,possessingasingle-stranded,nonseg-homodimerof124to140kDainitsunreducedformmentedRNAgenomeofpositivepolarity(7).Thevirion(75-78).Interestingly,thisprotein
7、ispresentonlyoncontainsfourmajorstructuralproteins:thenucleocap-somecoronaviruses,suchashumancoronavirussidprotein(N),thetransmembraneglycoprotein(M),(HCV)-OC43,hemagglutinatingencephalomyelitisthespikeglycoprotein(S),andthehemagglutinin/es-virusofswine,andturkeyent