冠状病毒Coronavirusl论文-1992 A Serologic Investigation for Coronavirus and Breda Virus Antibody in Winter Dysentery of Dairy Cattle in the North.pdf

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1、JVetDiagnInvest4:450-452(1992)AserologicinvestigationforcoronavirusandBredavirusantibodyinwinterdysenteryofdairycattleinthenortheasternUnitedStatesH.J.VanKruiningen,V.P.Castellano,M.Koopmans,L.L.Harris9Since1980,theamountofevidenceimplicatingabovinefectedcalf.BRV2is

2、usedforroutinediagnostictestsbecause8coronavirusasthecauseofwinterdysenteryhasbeenin-itismorestablethanBRVl.AdirectblockingELISAthat13creasing.Japaneseworkerswerethefirsttorecoveraco-hasbeenpreviouslydescribedwasusedtodetectantibody159,11ronavirusfromthefecesofacoww

3、ith“epizooticdiarrhea”;toBredavirus.2thisdiscoverywasfollowedbysimilarreportsfromBelgiumSerumneutralizationanti-coronavirusantibodytiterswere1andtheUnitedStates.Becausecoronavirus-likeparticlesdeterminedbyamethodsimilartothatemployedforrota-6andcoronavirusantigencan

4、befoundinthefecesofahighviruses.Nebraskastraincalfhooddiarrheacoronavirus(No.proportionofnormaldairycowsinsomeherdsduringthe874fromtheAmericantypeculturecollection)wasgrown3,4winterstablingseason,thesignificanceoftheseisolationsinMadinDarbybovinekidneycellsinthepres

5、enceof0.1%hasbeeninterpretedwithsomereservation.Serologicstudiespancreatinandintheabsenceofserum.Four-foldserialdi-50revealedhemagglutination-inhibitionseroconversiontoref-lutionsoftestserawerechallengedwith200-500TCIDforerencestrainsofbovinecoronavirusin59%ofaffect

6、edJap-90minutesat37C.Four-or5-day-oldcellmonolayersin15anesecattle;workersfromOhioreported4-foldorgreatermicroplatewells(growninEagle’sminimumessentialme-risesinserumneutralization(SN)titersin19of26animalsdium[EMEM]with10%fetalcalfserum)werewashedfree14(73%);andtheB

7、ritish,usingalatexagglutinationinhibitionofserum,inoculatedwith200µ1oftheserum-virusmixturestest,foundseroconversionin3of5affectedcattle?Were-(adsorbedat37Cfor120minutes),thenrinsedandincubatedcentlyreported63%seroconversionbyanenzyme-linkedfurther(withEMEMandpancre

8、atin).Thetestwasreadafterimmunosorbentassay(ELISA)method,in35sickanimals2-4days,andtheantibodytiterwasexpressedastherecip-16from8herdswith