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1、NOTEVirologyDetectionandIsolationofWinterDysenteryBovineCoronavirusCirculatedinKoreaduring2002–2004Jae-HoJEONG1),Gye-YeopKIM2),Soon-SeekYOON3),Su-JinPARK1),You-JungKIM1),Chang-MinSUNG1),Oh-JunJANG1),Sung-ShikSHIN1),Hong-BumKOH1),Bong-JooLEE1),Chai-YongLEE1),Mu
2、n-IlKANG1),Hyun-JinKIM1),Nam-YongPARK1)andKyoung-OhCHO1)*1)VeterinaryMedicalResearchCenter,CollegeofVeterinaryMedicine,ChonnamNationalUniversity,Gwangju500–757,2)CollegeofOrientalMedicine,DongshinUniversity,Naju,Chonnam520–714and3)PathologyDivision,NationalVet
3、erinaryResearchandQuarantineService,Anyang,Kyunggi430–824,SouthKorea(Received20April2004/Accepted7September2004)ABSTRACT.Althoughwinterdysentery(WD)hasbeensuspectedtooccurfrequentlyinKorea,todatetheexactepidemiologyofWDhasremainedunknown.Therefore,weinvestigat
4、edthecausativeagentsofWDbyusingelectronmicroscopy,ELISA,RT-PCR,andnestedPCRfrom97fecalsamplesof32WD-affectedherdscollectedfrom8provincesduring2002–2004.Thebovinecoronavirus(BoCV)wasconsistentlydetectedinallherdswithWD.Ofotherpathogens,onlycoccidianoocytswerein
5、consistentlybutconcurrentlydetectedwithBoCV.TenisolateswereidentifiedasBoCVbyimmuneelectronmicroscope,immunofluorescenttestandELISAwithantiserumtoBoCV,andRT-PCR.Fromtheseresults,itisconcludedthatWDcausedbyBoCVoccurredinrelativelyhighfrequencyandwaswidespreadin
6、Korea.TheresultsprovideimportantepidemiologicaldataforthecontrolandestablishmentofasurveillancesystemforWDinKorea.KEYWORDS:bovinecoronavirus,Korea,winterdysenteryprevalence.J.Vet.Med.Sci.67(2):187–189,2005Bovinecoronavirus(BoCV),amemberoftheCoronavir-ELISAempl
7、oyingmonoclonalantibodiestoBoCVascap-idaefamily,causesseverenewborncalvesdiarrhea(CD)tureantibodieswereusedtodetectBoCVinthefecalandisassociatedwithwinterdysentery(WD)inadultcattlesamplesaspreviouslydescribed[2,9–11,13].Todetectthe[5,10].WDischaracterizedbyasu
8、ddenonsetofdiarrheaBoCV-RNA,RT-PCRandnestedPCRwithspecificprimerthatrapidlyaffectsmanyadultcattleinaherdandhasbeenpairswereperformedwiththeextractedRNAfromfecalreportedinmanypartso
