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1、ArchivesofVirologyArchivesofVirology56,195--199(1978)©bySpringer-Verlag1978TheEffeetofAseorbieAeidonInieetionofChiek~EmbryoCiliatedTraehealOrganCulturesbyCoronavirusByJ.G.ATHERTON,C.C.KRATZING:andA~EFISHERDepartmentsofMicrobiologyandPhysiology,UniversityofQueensland,Brisbane,Australi
2、a.With1FigureAcceptedAugust10,1977SummaryChickembryotrachealorganculturesshowedincreasedresistancetoinfectionbyacoronavirusafterexposuretoascorbate,whilechickrespiratoryepitheliumandallantois-on-shellpreparationsshowednoincreaseinresistancetoinfectionbyaninfluenzavirusoraparamyxoviru
3、s.IntroduetionRecentlyitwasestablishedthatthemucoidsurface-filmoflungalveolaecontainsaveryhighconcentrationofascorbieacid.Whiletherearedifficultiesindeterminingtheascorbicacidcontentofthesurfacefilm,itwasestimatedtobebetween1.0and3.5mg/ml(16).Becauseitwasthoughtthattheseconcentration
4、smaybehaveasadefensivebarrier,systemsusingmonolayerculturesofchick-embryorespiratoryepithelium,allantois-on-shellandchick-embryoeiliatedtrachealorgan(CETO)cultureswereusedtostudytheeffectofascorbicacidonvirusinfection.ThevirusesusedweremembersoftheFamiliesOrthomyxoviridae(influenzaA)
5、,Paramyxoviridae(Newcastlediseasevirus,NDV),andCorona-viridae(avianinfectiousbronchitisvirus,IBV).MaterialsandMethodsRespiratoryepitheliumcultureswerepreparedeitherin"Falcon"tissuecultureflasks(30ml)orin"Linbro"96cuppolystyrenetissueculturemicroplatesIS-FB-96-TC.Allantois-on-sbellpre
6、parationsweremadebythemethoddescribedbyFAZE~:ASand%V]c[ITE(6).CETOcultureswerepreparedbythemethodofCttER~¥andTAYLOR-ROBI~SO~(4)withminormodifications.Thetracheaweredissectedoutfrom20day-oldchick-embryosandcuttransverselyintorings1mmthick.Afterwashing,theringswereplacedindividuallyint
7、otubescontaining3mlofEagle'sbasalmedium(BME)plusglutamineandantibiotics,bufferedtopH7.4.Fullcilialactivitycouldbeobserved13Arch.Virol.56/30304-8608/78/0056/0195/$01.00196J.G.A~HER~ON,C.C.Km~TzINC,,andANNEFISI-IEt¢:microscopicallyforupto21dayswhentheseculturesweremaintainedat37°C,with
8、mediumchange
