冠状病毒Coronavirusl论文-1977 Experimental infection of adult axenic rats with Parker_s Rat Coronavirus.pdf

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1、ArchivesofVirologyArchivesofVirology54,345--352(1977)©bySpringer-Verlag1977ExperimentalInfeetionofAdultAxenieRatsWithParker'sRatCoronavirusByP.N.BHATTandR.O.JACOB¥SectionofComparativeMedicine,YaleUniversitySchoolofMedicine,NewHaven,Connecticut,U.S.A.With5F

2、iguresAcceptedMarch31,1977SummaryThepathogenesisofParker'sRatCoronavirus(PRCV)wasstudiedinaxenieCDrats.Threetofour9to10weekoldratswereeuthanizeddailyforeightdaysafterintranasalinoculation.Ratsremainedfreeofclinicaldisease.Viruswasre-coveredfromthenasophary

3、nxandtracheaaftertwenty-fourhoursandfromthelungbydaythreebutwasnotdetectedinrespiratorytract,aftersevendays.Viralantigenwasdetectedbyindirectimmunofluoresecnceinthemueosalepi-theliumofupperrespiratorytractandinpulmonaryalveolarseptaefromdaytwotosixpostinoc

4、ulation.Acuterhinitisdevelopedbydaytwoandwasassociatedwithmildfocalnecrosisofrespiratorymucosalepithelium.Mildnonsuppurativetracheitisandmultifocalinterstitialpneumoniaappearedbydayfiveandper-sistedthroughdayeight.Dacryoadeaitisdidnotoccur,sialoadenitiswas

5、detectedinonlythreeratsandviruswasrecoveredfromonlyonesubmaxillarysalivarygland.ThisexperimentindicatesthatPRCVcanbeaprimarypathogenfortherespiratorysystemofadultrats.Incontrasttosialodacryoadenitis(SDA)virusthetropismofPRCVforsalivaryandlacrimalglandsislo

6、w.IntroductionCoronavirusinfectioniscommoninlaboratoryratsandtwoantigenicallyrelatedviruses,sialodacryoadenitisvirus(SDAV)andParker'sratcoronavirus(PRCV),havebeenisolatedfromnaturally-infectedrats(2,7).SDAVcausessevereself-limitingsialodacryoadenitisinnatu

7、rally-infectedorexperimentally-inoculatedadultrats(4,5)andrecentevidenceindicatesthatSDAVisassociatedwithnaturally-occurringkeratoeonjunetivitisinrats(6).PRCVwasoriginallyisolatedfromthelungsofratswithahighincidenceofcomplement-fixing(CF)23*34:6P.N.BHA~'Ta

8、ndR.0.JACOB¥:antibodytomousehepatitisvirus(MHV)(7).IncontrasttoSDAV,naturalin-fectionwithPRCVisreportedlyasymptomaticinadults,butexperimentally-inoculatedneonatesdevelopedlethalinterstitialpneumonia(7).Thisre