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1、VeterinaryMicrobiology,2(1977)73--8173©ElsevierScientificPublishingCompany,Amsterdam--PrintedinTheNetherlandsPHYSICO-CHEMICALPROPERTIESOFCALl!~:,IARRHEACORONAVIRUSK.SATO,Y.INABA,H.KUROG~,E.TAKAHASHI,Y.ITO,Y.GOTO*,T.OMORIandM.MATUMOTO**NationalInstituteofAnimal
2、Health,Kodaira,Tokyo(Japan)*NationalVeterinaryAssayLaboratory,Kokubunji,Tokyo(Japan)**KitasatoInstitute,Minato-ku,Tokyo(Japan)(Received17November1976)ABSTRACTSato,K.,Inaba,Y~,Kurogi,H.,Takahashi,E.,Ito,Y.,Goto,Y.,Omori,T.andMatumoto,M.,1977.Physico-chemicalpro
3、pertiesofcalfdiarrheacoronavirus.Vet.Microbiol.,2:73--8LReplicationofcalfdiarrheacoronaviruswasnotinhibitedby5-iodo-2'-deoxyuridine,indicatingthatthevirIIsisanRNAvirus.Sensitivitytoetherandchloroformindicatedthatthevirusisenveloped,andthiswasconfirmedbyelectro
4、nmicroscopicobservationofthevirion.Theviruswasreadilyinactivatedbytrypsinandsodiumdeoxycholate.Theviruswaslabileat50°Cindilutedmedium,butreadilystabilizedinthepresenceofMgCI~.ItwasstableatpH5and7,whileaslightlossofinfectivitywasobservedatpH3.Theviruswasreadily
5、filteredthroughmembranefiltersof200and100-nmporesizes,butnotthrough50-amfilters.ThebuoyantdensityofthevirioninCsCIw~sestimatedtobe1.25g/m].INTRODUCTIONCalfdiarrheacoronaviruswasrecentlyrecoveredfromthefecesofcalveswithneonataldiarrheaandprovedtobeacausativeage
6、ntofthedisease(Stairetah,1972;Mebusetal.,1972,1973aandb;Sharpeeetal.,1976).Thevirusmultipliedinbovineembryonickidneycellcultures,butfailedtoinducereadilyrecognizablecytopathiceffect(Mebusetal.,1973a).Recent-lywehaveobsercedthatthevirusreadilyreplicatesandinduc
7、esamarkedIcytopathiceffectinculturesofacontinuouscellline,BEK-1,derivedfrovllbovineembryonickidney.Thisobservationhasprovidedasensitive,practicalassaymethod(Inabaetal.,1976),wherebythevirusanditsdiseasecannowbestudiedmoresystematically.Thepresentstudywasundert
8、akentolearnsomeofthephysico-chemica]propertiesofthevirus.MATERIALSANDMETHODSCellcultures.--BEK-1cellsweregrownasdescribedpreviously(lnabaetal.,741976).ThegrowthmediumusedwasEagle's
