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1、PROCEEDINGSOFTHESOCIETYFOREXPERIMENTALBIOLOGYANDMEDICINE155,143-148(1977)DetectionofCoronavirusInfectionofManbylmmunofluorescencel(39761)AkNOLDS.MONTOANDLENOREM.RHODESDepartmentofEpidemiology,SchoolofPublicHealth,TheUniversityofMichigan,AnnArbor,Michigan4810
2、9Sinceprimaryisolationofthehumanco-initiallyinoculatedintramuscularlywith1.Oronavirusesisdifficulttoaccomplish,muchmlofthevirusinanequalvolumeofincom-reliancehasbeenplacedonserologicmeth-pleteFreund'sadjuvant.At3weeks,1.Omlodsforidentificationofinfections(1)
3、.Theofthehomologousviruswasgivenipandtechniquesthatgenerallyhavebeenem-thefinalbloodspecimenswereobtained10ployedarecomplementfixation(CF)anddayslater.Titersoftheantiserawere1:64hemagglutinationinhibition(HI)(2,3).for229Eand1:128forOC43.BeforeuseinHowever,ce
4、rtainproblemsofsensitivitytheFAtest,theseserawereabsorbedwithandspecificityareinherentinthesemethodstheparticularnoninfectedcellstoridthemanddifficultyininterpretationofresultscanofnonspecificreactivity.Inaddition,nonin-sometimesberesolvedonlybyuseofthefarfe
5、ctedcellcontrolswereusedinalltests,morecomplicatedneutralizationtest(2,4,andwereuniformlyfoundtobenegative.5).Anadditional,moresimpletestwouldHumanspecimenswereobtainedfromthethereforebeofgreatvalue.ThefluorescentcollectionoftheTecumsehStudyofRespi-antibody(
6、FA)techniquehasrarelybeenratoryIllnesses.Theseserahadbeencol-employedtodetecthumancoronavirusanti-lectedfromallthoseundersurveillanceat6bodyalthoughitdidplayaroleintheorigi-monthlyintervals.Theparticularpairsofnalidentificationoftheorgancultureiso-serausedha
7、dbeenfoundbyotherproce-lates(6).Methodshavenowbeendevel-durestoexhibitafourfoldriseincoronavi-opedforthemeasurementbyimmunofluo-rusantibody.rescenceofantibodyagainsthumancoro-Fluorescentantibodytechnique.CulturesnavirusOC43and229E.TheprocedureisofWI-38wereco
8、mmerciallyobtainedhereindescribedandresultsarecompared(HEMLaboratories)andcellswerepropa-withthoseobtainedwithCF,HI,andneu-gatedseriallyinEagle'sminimumessentialtralization.medium(MEM)supplement
