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1、当归多糖的分离、纯化及分析鉴定作者：商澎杨铁虹贾敏梅其炳赵文明曹之宪赵德化【关键词】当归/分离和提纯关键词：当归/分离和提纯;当归/分析;多糖摘要：目的从新鲜中国当归[Angelicasinensis(Oliv)Diels]中分离纯化出多个多糖组分，并对其理化性质进行分析鉴定.方法采用水煮一乙醇沉淀法从新鲜当归中提取当归总多糖AP-0;AP-0用80g・kg-l十六烷基三甲基漠化鞍(CTAB)沉淀得到当归多糖亚组分AP-1;CTAB处理后的上清用10g・kg-lH3B03,2mol・L-INaOH处
2、理得到当归多糖亚组分AP-2;所剩上清，用乙酸处理后,再用乙醇沉淀，得到当归多糖亚组分AP-3.总糖含量测定采用改良苯酚-硫酸法;蛋白质含量测定采用ServaBlue-G染料结合法;糖醛酸含量测定采用间轻基连苯法•采用新华中速滤纸纸色谱和硅胶G薄层色谱分析多糖各组分的单糖组成•高效凝胶过滤色谱和高效离子交换色谱分析多糖各组分的Mr分布和电荷性质.红外光谱法分析多糖各组分的糖甘键构成•结果AP-0总糖970g・kg-1,其中糖醛酸210g・kg-l，蛋白质30g・kg-l;AP-l总糖970g
3、539;kg-l,其中糖醛酸172g・kg-l,蛋口质30g・kg-l;AP-2总糖830g・：kg-l,其中糖醛酸257g・kg-l，蛋白质170g・kg-l;AP-3总糖970g・kg-l,其中糖醛酸86g&#l2539;kg-1，蛋白质30g&#l2539;kg-1.AP-0,AP-lMP-2和AP-3主要由葡萄糖，阿拉伯糖和少量糖醛酸组成・AP-0,AP-1,AP-2和AP-3分别由5种不同分子量多糖部分组成;在电荷特性上又可分别分为4个不同部分•红外
4、光谱提示4种多糖的糖链构型为a-D-葡萄毗喃型.结论当归多糖的分离纯化方法有效、实用;得到的AP-0,AP-1,AP-2和AP-3为具有不同理化性质的多糖组分.Keywords:angelicasinensis/isolation&purification;angelicasinensis/analysis;polysaccharidesAbstract:AIMToisola.teandtopurifysomepolysa.ccha-ridesfractionsfromAngelicasinensis(Oliv.)Dielsandt
5、oan-alyzeandtodeterminethecharactersofeachfraction.METHODSHotwaterextractingandethanolprecipitatingmethodwasemp1oyedtoisolatetotalpolysaccharide(namedAP-0)fromfreshrootsofAngelicasinensis・BytreatingAP-Owith80g・kg-lCetyltrimethylammoniumBromid(CTAB),theAP-lwasobtaine
6、dfromtheprecipitate・AP~2wasobtainedfromtheprecipitatebytreatingthesupernateleftfromprecedingstepwithlg・kg-1H3B032mo1・LNaOH.Aftertreatingtheleftsupernatewithacetaticacidandprecipitingwithethanol,AP-3wasobtained・Theamountsoftotalcarbohydrates,proteinsanduronica
7、cidswei'edetenninedwithiniproveclphenol-sulfuricacid,ServaBlueGdyebindingandm-hydroxyldiphenylmethods,respec-tively.Themonosaccharidescontainedineachpolysaccha-rideswereanalysedbyusingpaperchromatographyandthin~layerchromatography・Highperformancegelfiltrationchro-matograph
8、yandhighperformanceanion-exchangechro-matographywereemployedtodeterminetherelativemolecu~
