巨噬细胞炎性蛋白 1α在病毒性心肌炎小鼠中的表达及意义.doc

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1、6.对照组MTP-la蛋白呈弱表达，心肌炎组小鼠于感染后7dMTP-la表达最明显，15〜30d其表达逐渐下降，但仍高于对照组（PV0.01或0.05）,而3d与对照组比较，差异无显著性（P>0.05）7.心肌MIP-la蛋白表达水平与心肌病理积分的相关性：心肌炎组心肌MIP-la蛋白表达水平与心肌病理积分呈显箸正相关（尸0.82,P<0.05）；8.血清MIP-la浓度与心肌病理积分的相关性：心肌炎组小鼠血清MIP-1a浓度与心肌病理积分呈显著正相关（尸0.89,户<0.05）。9.心肌炎组7〜30d各时点心肌IL・1[3、TNF-a含量较对照组显著升高（P<0.01）,但3d与对照组比较,

2、差异无显著性（？>0.05）。结论1.MIP-la可能参与VM发病过程，其机制可能与促进炎症细胞向心肌浸润及增加IL-ip>TNF-a分泌有关；2.血清MTP-la浓度能反映心肌炎的严重程度，可作为心肌炎病情判断的一个血清学指标。关键词：巨噬细胞炎性蛋白Ta；病毒性心肌炎；白细胞介素-1卩；肿瘤坏死因子-aExpressionandSignificanceofMacrophageInflammatoryProtein-lainMurineViralMyocarditisAbstractObjectiveMacrophageinflammatoryprotein-1a(MIP-1a)9amemb

3、erofCCsubfamilyofchemokines,canspecificallyinducechemotaxisofinflammatorycellsincludingmonocytes,lymphocytesandneutrophils,therebybeinginvolvedinpathogenesisofavarietyofinflammatorydiseases.TheaimofthepresentstudywastoinvestigatethekineticfeaturesofMIP-laexpressioninmicewithviralmyocarditis(VM),andi

4、tsrolesinthepathogenesisofVM.Method：SeventyfiveBalb/cmicewererandomlydividedintotwogroups:themyocarditicgroupandacontrolgroup.Miceinthemyocarditicgroup(n=65)wereinoculatedintraperitoneallywith0」mLof1X102TCID50coxsackievirusB3(CVB3)dilutedinEagletminimalessentialmedium(EMEM)solution.Controlmiceweretr

5、eatedwith0」mLofEMEM・Afterweighingbodyweight(BW),10infectedmiceweresacrificedondays3,7,15and30postinoculation,andthecontrolmicewerekilledonday30postinoculation.Theheartswereremovedandweighedtocalculateheartweight(HW)/BW.Bloodwascentrifugatedtoobtainserum.Changesofmyocardialhistopathologywereexaminedt

6、hroughHEstain.ExpressionofMIP-lamRNAandproteininthemyocardiumwereexaminedbyRT-PCRandwesternblotting,respectively.SerumMIP-laconcentrations,myocardialinterleukin-1卩andtumornecrosisfactor-a(TNF・a)contentsweredetectedusingenzymelinkedimmunosorbentassay(ELISA).CorrelationanalysesbetweentheexpressionofMI

7、FproteinorscrumMIP-laconcentrationandmyocardialhistopathologicscoreswereinvestigated.Results1・Themortalityinmyocarditicgroupwas38.46%(25/65).Miceincontrolgrouphadnodeath.2.Myocardialmorphologywasnorma