癫痫小鼠模型记忆减退的电生理学研究

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1、上海交通大学硕士学位论文癫痫小鼠模型记忆减退的电生理学研究姓名：张坍中请学位级别：硕士专业：神经病学指导教师：陆钦池20090401ALTERATIONOFELECTROPHYSIOLOGYINEPILEPSYMICEMODELWITHIMPAIRMENTOFMEMORYABSTRACTStatusepilepticus(SE)inanimalmodelsofpilocarpine(PILO)-inducedseizurehasbeenshowntoproducedeficitsinspatiallearningandmemory.Intheexpe

2、rimentsasreportedherein,seizuresinducedbyinjectionoflithiumchloride-pilocarpine(LiCl-PILO)in7-weekoldmicewerefoundtoreducecontextualfearmemoryat6weeksaftersuchinjection.Correspondingly,theinductionoflong-termpotentiation(LTP)inhippocampalCAIneuronswasalsoimpaired.Thesuppression

3、ofLTPwasaccompaniedbyanincreaseinpairedpulsefacilitationratiooftheslopeofexcitatorypostsynapticpotentials(EPSPs),whiletheinput-outputrelationshipsofEPSPsdidnotchange,suggestingapresynapticlocusoftheaction.KEYWORDS:epilepsy,LiCl-PILO,contextualfearmemory,hippocampus,long-termpot

4、entiation图片目录图1.海马横断面的突触联系5图2.海马CA1区Scaffer侧支EPSP记录和LTP现象14图3・LTP的诱导过程17图4.LiCl-PILO癫痫持续状态模型制作流程图22图5.场景恐惧记忆测试的操作过程23图6.场景恐惧实验设备24图7.实验所得海马切片实体照片26图8・电生理实验装置系统28图9.LiCl-PILO诱发的痫性发作损伤场景恐惧记忆的形成33图10.LiCl-PILO诱发的痫性发作对LTP形成的影响36图11.24h组输入/输出检测38图12・6w组输入/输出检测39图13・LiCl-PILO诱发的痫性发作对

5、PPFratio的影响41图14.注射生理盐水后24h对照组的LTP低于正常对照组45缩写及符号缩略词英文全称中文名PILOPilocarpine匹罗卡品LiClLithiumchloride氯化锂SEStatusepilepticus癫痫持续状态KAKainicacid海人酸CSConditioningstimuli条件性刺激USUncongditiongingstimuli非条件性刺激LTPLong-termpotentiation长时程增强LTDLong-termdepression长时程抑制GABAy—aminobutyricacidY•氨基

6、丁酸NMDAN-methyl-D-aspartateN■甲基・D■天冬氨酸AMPAa-amino-5-methyl-3-hydroxy-4-isoxaz*氨基・3■轻基・5■甲olepropionicacid基・4•异啤丙酸TLETemporallobeepilepsy聂页叶癫痫DGDentategyrus齿状回PPPerfbrantpath穿通支ECEntorhinalcortex内嗅皮质MFMossyfibre苔蘇纤维SCSchaffercollaterallpathwaySchaffer侧支ACAssociationalcommissuralp

7、athway联合支EPSPExcitatorypostsynapticpotential兴奋性突触后电位ACSFArtificialcerebrospinalfluid人工脑脊髓液PTXPicrotoxin印防已毒素DMSODimethylsulfoxide二甲基亚砚HFSHighfi-equencystimulus高频刺激I/OInput/outputrelationships输入/输出PPFPairedpulsefacilitation双脉冲易化PTZPentylenetetrazol戊四氮mGluRsMetabotropicglutamater

8、eceptors代谢性谷氨酸受体BKCalcium-activatedpotassiumchannels钙激