水溶性低聚壳聚糖的工艺探究

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1、毕业论文题甘名称水溶性低聚壳聚糖的工艺探究学院文理学院专业/班级应用化学12101学生姓名郝建涛班级学号41312133指导教师陈琼摘要壳聚糖作为一种化学产品，给人们的衣食住行已带來了很人到好处。为了确定以虾蟹壳为原料制取壳聚糖的改良新工艺的最佳条件，于是通过单因素实验选収实验因素与水平的探讨和研究，其工艺条件为：10%盐酸溶液和15%的助剂乙酸于常温水浴持续搅拌4h进行脱钙，以10%氢氧化钠溶液于90°C的水浴中持续搅拌6h进行脱蛋白；以10%过氧化氢于常温浸泡2h进行了完全的脱色素过程，此吋便可得到可溶于0.lmol/L盐酸的甲壳素.根据Box-Benhnken的屮心组合实验

2、设计原理，在单因索试验的基础上采用三因素三水平的响应血分析法。将制取的甲壳素于三颈烧瓶屮加入31%MM化钠溶液,55%助剂乙醇溶液于110°C的水浴中持续搅拌8h进行了脱乙酰的过程，得到不溶于0.lmol/L盐酸的壳聚糖.用甲基红指示剂标定法测定脱乙酰度•依据回归分析确定各丄艺的影响因子，以壳聚糖的脱乙酰度为响应值做响应而和等值曲线图•最后将制取的壳聚糖在一定条件下进行降解得到水溶性低聚壳聚糖.关键词:甲売素；売聚糖；脱乙酰度；响应面AbstractChitosanasakindofchemicalproduct,tothedailylifeofthepeoplehasbroug

3、htgreatbenefits・Inordertodetenninewithshrimpandcrabshellasrawmaterialforthepreparationofchitosanmodifiedprocess,theoptimalconditions,andthroughthesinglefactorexperimentselectedexperimentalfactorsandlevelsofthediscussionandtheresearch,thetechnologicalconditions:10%hydrochloricacidsolutionand15

4、%aceticacidadditivesinwaterbathatroomtemperaturecontinuousmixing4hweredecalcified,with10%sodiumhydroxidesolutionin90degewaterbathincontinuousstirring6hforproteinremoval;with10%hydrogenperoxideatroomtemperaturesoaking2hwerecompletelydepigmentationprocess.Thenwecangetdissolvedin0」mol/Lhydrochlo

5、ricacidarmourShellelement.Accordingtotheboxbenhnkencentralcompositeexperimentaldesignprinciples,onthebasisofsinglefactorexperimentwiththreefactorsandthreelevelsofresponsesurfaceanalysismethod・Thepreparationofchitininathreeneckedflaskwith31%sodiumhydroxidesolution,55%additiveethanolsolutiontol

6、lOdegcwaterbathwhisking8hthedeacetylationprocess,dissolvedin0.1mol/Lhydrochloricacidchitosan.Withmethylredindicatorcalibrationmethodforthedetenninationofdegreeofdcacctylation.Accordingtotheregressionanalysistodeterminethefactorsafleetingtheprocess,withchitosandeacetylationdegreeasresponsevalu

7、eofresponsesurfaceandcontourcurvediagram.Finally,thepreparedchitosaninaThedegradationofwatersolublechitosanwasobtainedundercertainconditions・Keywords:Chitin;chitosan;degreeofacetylation;responsesurface第一章综述1.1甲壳素的发展史甲壳素（Chitin）是一种天然高分子物质，属于多糖