蔓荆子活性成分vitexicarpin诱导K562细胞凋亡的机制

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1、蔓荆子活性成分vitexicarpin诱导K562细胞凋亡的机制王海燕「2,蔡兵J崔承彬'，张冬云-杨宝峰$(I.天津生物医药研究所.天津300384；2.哈尔滨医科大学药理教研室，黑龙江哈尔滨150086；3.军事医学科学院毒物药物研究所.北京100850)摘要：目的探讨vitexicarpin对人癌细胞增殖的抑制活性及其抗癌机制：方法以SRB法进行细胞毒测定，观察细胞的形态学变化并用流式细胞术分析癌细胞的凋亡率.DMA琼脂糖凝胶电泳检测凋亡细胞的DNA梯带，以Westernblotting法进行蛋白质检测。结果Vitexicarpin对K562等4种人癌细胞表

2、现了较强的增殖抑制活性。处理后的K562细胞表现岀典型的凋亡形态特征、出现了剂量依赖性增加的亚二倍体峰并呈现出典型的DNA梯形条带；PARP和easpase-3被剪切、胞浆中细胞色素c增高、表达减少、Bax表达未见明显变化：结论Vitexicarpin通过激活线粒体调控的凋亡通路诱导K562细胞凋亡。关键词：蔓荆子；vitexicarpin；抗肿瘤活性；细胞凋亡中图分类号：R282.71；H963文献标识码：A文章编号：0513-4870(2005)01-0027-05Vitexicarpin,aflavonoidfromVitextrifoliaL.,induc

3、esapoptosisinK562cellsviamitochondria-controlledapoptoticpathwayWANGHai-yan'*2,CAIBing',CUICheng-bin3*,ZHANGDong-yun',YANGBao-feng2(/.TianjinInstiluteforBiomedicinalResearch,Tianjin300384,China；2.PharmacologyDepartmentHarbinMedicalUnii^rsity,Harbin150086,China;3.BeijingInstituteofPhar

4、macologyandToxicology.Beijing1008501China)Abstract：AimToinvestigatetheinhibitoryeffectofvitexicarpinontheproliferationofhumancancercellsanditsmechanismofaction.MethodsTheinhibitoryeffectofvitexicarpinontheproliferationofhumancancercellswasevaluatedbytheSRBmethodanditsapoptosis-inducin

5、geffectwasdemonstratedbymorphologicalobservationunderlightmicroscope,flowcytometricanalysisandagarosegelelectrophoresis.TheproteinsrelatedtoapoptosiswereexaminedbyWesternblottinganalysis.ResultsVitexicarpinsignificantlyinhibitedtheproliferationofhumancancercells,A2780,HCT-15,HT-1080an

6、dK562,withtheIC^valuesof(19.1±2.4)pjnol・I,"forA2780(48h),(0.66±0.10)p,mol・L"forHCT-15(48h),(0.44±0.06)pnol•L1forHT-1080(48h)and(0.28±0.14)jxmol・L_,forK562(24h)・Thecellstreatedwithvitexicarpinshowedcharacteristicmorphologytypicalforapoptosisandgavedose-dependentsub-G0/G

7、peakintheflowcy

8、tometricanalysisandDNAladderonagarosegelelectrophoresis.InWesternblottinganalysis,thecleavageofPARPandcaspase-31thereleaseofcytochromecfrommitochondriaintothecytosol,thedecreaseofBcl-2expressionlevel,andthedownregulationoftheratioofBcl-2/BaxexpressionlevelwereexaminedintheK562cellstr

9、eated