cdks抑制剂对卵巢癌细胞株skov3细胞周期和凋亡影响

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1、EffectsoftheinhibitorofCDKsoncellcycleandapoptosisintheAbstractovaraincancercelllineSK0v3Background：Ovariancanc盯isoneofthegynecologicaltumorandisagreatthreatofwomen’slife．IthasbeenprovedthatcyelinsandCDKsareover-expressedinovariancanGerwhichispossiblyrelative谢t

2、lltheprognosisandthedrugresistance．Roscovitine，asaninhibitorofCDKs，hasbeenwidelystudiedinitsanti—tumoreffect．Itmayalsodown-regulateX-linkedinhibitorofapoptosisprotein．ButtherearefewstudiesaboutitseffectonovarianCancerobject：ToinvestigatetheeffectsofRoscevitineO

3、ntheapoptosis．thecellcycleandtheregulationofXIAPinovariancancercelllineSKOV3．Methods：SKoV3cellsweretreatedwithRoscovitineatdifferentcencentrations(5、lO、25、50and100umol，L1．Atier24、48and72hours,MTTassaywasusedtoevaluatetheinhibitoryeffectonthecells．Atiertreatedth

4、ecellswithdifferentconcentrationsofRoscovitine,cellswerecollectedatdifferenttimeandtheapoptosisandthecellcyclewereexaminedbyFlowCytometryusingAnnexinv-FITC／PIandPI．WesternblotwasusedtodetecttheexpressionchangesofXIAP．Results：1．Comparedwimtheuntreatedgroupandthe

5、menslruumgroup．thegrowthofSKOV3cellswasinhibitedbyRoscevitineindoseandtime-dependentmanner8．AtiertreatedthecellswiIllRoscovitineattheconcentrationsof25、50and100umoFLfor48hours．theinhibitoryrateswere22．93％、65．58％、78，64％(P<0．05)．ButtheexposuretoRoscovitinelongert

6、han48hoursathighconcentrations(50and100umol／L)didnotincreasetheinhibitoryrate．2．Comparedwiththeuntreatedgroupandthemenstruumgroup，theapoptosis5ofSKOV3cellsWaSinducedbyRoseovifineindoseandtime—dependentmallllers．Aftertreatedwith25umoI／LRoscovitinefor24、48and72ho

7、urs，theapoptosisrateswem7．56％、28．31％、42．15％(P<0．01)．3．Comparedwiththeuntreatedgroupandthemenstruttmgroup，theproportionoftheeellofGo／zphasewasincreasedandSphaseWaSdecreasedattertreated谢thRoseovitine(10umol／Land25umol／L)(P

8、ndentmannerandtherewasnotanychangeintheproportionoftheG2Mphase．4．Comparedwiththetmlreatedgroupandthemenstruumgroup，theexpressionXIAPinSKOV3cellsWaSmarkedlydecreasedattertrea