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1、S100A16在饮食诱导肥胖大鼠中的作用研究［摘要］目的：探讨S100A16蛋白对体重增加过程的影响，进一步研究S100A16蛋白在肥胖及肥胖相关疾病的发生发展过程中的作用。方法：正常8周龄大鼠随机分为正常饮食组(NF,n=10)和高脂饮食组(HF,n=10),建立饮食诱导的肥胖(DIO)大鼠模型，喂食14周时进行腹腔葡萄糖耐量试验(IPGTT)及胰岛素释放试验(IRT),喂食16周处死后称量皮下及内脏脂肪重量，采用HE染色方法(hematoxylinandeosinstaining,HE)观察肝脏脂肪变性程度，放射
2、免疫分析法检血糖，血清胰岛素，尿酸等血清生化指标，同时应用Westernblotting方法检测脂肪组织中S100A16及糖脂代谢相关转录因子的蛋白表达。结果：DIO组大鼠的体重，内脏脂肪明显高于正常组，血清总胆固醇和尿酸DIO组高于正常组但糖耐量和胰岛素释放低于正常组，westernblotting显示DIO组大鼠脂肪和肝脏组织中S100A16,PPAR-y,C/EBP-a的蛋白表达均高于正常组。结论：高脂饮食可上调S100A16及相关转录因子的表达；S100A16的高表达可促进脂质生成及肥胖发生，并对机体的胰岛素
3、敏感性产生负性影响。［关键词］胰岛素敏感性，肥胖，大鼠模型，S100A16,转录因子［中图分类号JR587.1［文献标识码］ATheroleofS100A16inratswithdiet-inducedobesityObjective:ToinvestigatetheeffectofS100A16onweightgainprocess,thedevelopmentofobesityandobesityrelateddiseasesoccu匚Methods:Ratsaged8weekswererandomlydivid
4、edintonormaldietgroup(NF,n=10)andahigh-fatdietgroup(HF,n=10),providingdietfor16weekstoestablishadietinducedobesity(DIO)ratmodel.Whenfeedingat14weeks,ratswereprocessedintraperitonealglucosetolerancetest(IPGTT)andinsulinreleasetest(IRT).Whenratswereexecutedafter1
5、6weeks,weightedsubcutaneousandvisceralfatweight.ThenweusedHEstainingmethod(hematoxylinandeosinstaining,HE)toobservetheliversteatosisdegree,radiationimmunityanalysistocheckbloodsugar,insulin,serumuricacid,serumbiochemicalindicator,andWesternblottingtostudytheexp
6、ressionofS100A16andglucolipidmetabolismrelatedproteinexpressionoftranscriptionfactorsinliverandadiposetissue.Results:DIOgroupratsweight,visceralfatissignificantlyhigherthannormalgroup・TheserumtotalcholesterolanduricacidinDIOgroupwashigherthannormalglucosetolera
7、nceandinsulinreleaseislowerthanthenormalgroup.WesternblottingshowedthatinliverandfiattissueofDIOgroup,S100A16,PPAR-y,C/EPB-aexpressionweresignificantlyhigherthanthoseofnormalgroup.Conclusion:HighfatdietcanincreasetheexpressionofS100A16andrelatedexpressionoftran
8、scriptionfactors;S100A16overexpressioncanpromotelipidgeneratedandresultinanegativeimpactoninsulinreleaseandinsulinsensitivity.Keywords:Insulinsensitivity;obese;ratmodel;S100
