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1、中国启东肝癌高发区肝癌中AKRlC2基因异常表达及其在肝癌发生中作用生物化学与生物物理进展Prog.Biochem.Biophys.2003;30(6)TheEffect0fAKR1C2GeneonandItsAbnormalExpressionCarcinomaFromQidong,Hepat0carcin0genesisinHepatocellularChina,aLiverCancerHighRiskAreaLUD0ng.D0ng'),ZHANGXi.Ran),CA0Xiang.R0ng)("TheCollegeofLifeScience,NanjingNorm
2、alUniversity,Nanjing210097,China;QidongLiverCancerInstitute,Qidong226200,China)AbstractAbnormalAKR1C2expressionhasbeenobservedinmanymalignanthumantumors.butiIsrelationshipwitllhepatocellularcarcinoma(HCC)isnotwellunderstoodsofar.InordertoevaluatehepatocarcinogeniceffectofAKR1C2geneandth
3、esignificanceofitsabnormalexpressioninhepatocellularcarcinoma,AKR1C2geneisanalysedbypreparingrabbitanti—humanAKR1C2polyclonalantibody.constructingofAKR1C2frarneshiftmutantandexploringRT—PCR.insituhybridization.immunohistochemistry.Westernblot.Northernblot.cDNAexpressionmicroarray,co—imm
4、unoprecipitationandtlletumorigenicityassayvivoandinvitroetc.AKR1C2geneexpressionandiIseffectswasanalyzed.including68pairsofHCCspecimensandiIsadjacentpara—canceroustissues.8casesofnormallivertissuesandQGY7703cellline.ResulIsshowedAKR1C2expressionwasup—regulatedamongthesepatients.whencomp
5、aredwitlltlloseofpara—cancerousandnormallivertissues.Over.expressionofAKR1C2isalsofoundtobecorrelatedwitllhighmetastasispotentialityofHCC.AKR1C2overexpressionstimulatesDNAsyntllesis.apoptosis.growthinsoftagarandpromotetumorformationandleadtoexpressiondirencesoftumorgenes.AKR1C2mediatedt
6、lleNF—KB—dependentresistanceof9GY7703cellstoanti—faskilling.IntracellarbindingofAKR1C2andCdk4wasfound.AbnormalexpressionofAKR1C2genemaycontributetotlleoccurrence.advancementandinvasivenessofHCCfromQidong.China.alivercancerhighriskarea.Keywordslivertumor,AKR1C2gene,metastasis.invasivenes
7、sgeneexpression,apoptosis,tumorigenicityassaysHepatocellularcarcinoma(HCC)isquitecommoninChina,especiallyinQidong,JiangsuProvince.whereHCCmortalityratewas80/1ooo0O.Inrecentyears,greatprogresseshavebeenmadeinthepreventionandtreatmentofHCC,butthemaiorproblemisthehighmortalityrate
